Chronic cannabis use is associated with altered monocyte phenotype, immune response, and depression in physically active individuals.

Autor: Lisano JK; Department of Kinesiology, Nutrition, and Dietetics, University of Northern Colorado, Greeley, CO, USA., Kisiolek J; Department of Kinesiology, Nutrition, and Dietetics, University of Northern Colorado, Greeley, CO, USA., Flores V; Department of Kinesiology, Nutrition, and Dietetics, University of Northern Colorado, Greeley, CO, USA., Smoak P; Department of Kinesiology, Nutrition, and Dietetics, University of Northern Colorado, Greeley, CO, USA., Pullen NA; School of Biological Sciences, University of Northern Colorado, Greeley, CO, USA., Stewart LK; Department of Kinesiology, Nutrition, and Dietetics, University of Northern Colorado, Greeley, CO, USA.
Jazyk: angličtina
Zdroj: Canadian journal of physiology and pharmacology [Can J Physiol Pharmacol] 2023 Jun 01; Vol. 101 (6), pp. 316-326. Date of Electronic Publication: 2023 Mar 03.
DOI: 10.1139/cjpp-2022-0451
Abstrakt: Introduction: This study evaluated depression, monocyte phenotype, and immune function in physically active cannabis users. Methods: Participants ( N  = 23) were classified as either cannabis users (CU, n  = 11) or non-users (NU, n  = 12). White blood cells isolated from blood were analyzed for co-expression of cluster of differentiation 14 and 16 using flow cytometry. Lipopolysaccharide (LPS) was cultured with whole blood and assessed for interleukin-6 and tumor necrosis factor-α (TNF-α) release. Results: The percentage of white blood cells classified as monocytes was not different between groups; however, CU had a significantly greater percentage of monocytes classified as intermediate ( p  = 0.02). When standardized per milliliter of blood, CU had significantly greater numbers of total monocytes ( p  = 0.01), classical monocytes ( p  = 0.02), and intermediate monocytes ( p  = 0.01). Intermediate monocytes per milliliter of blood were positively correlated to the number of times CU used cannabis per day ( r  = 0.864, p  < 0.01) and Beck Depression Inventory-II (BDI-II) score ( r  = 0.475, p  = 0.03), which was significantly greater in CU (5.1 ± 4.8) compared with NU (0.8 ± 1.0; p  < 0.01). CU released significantly less TNF-α per monocyte in response to LPS. Conclusions: CU had altered monocyte phenotypes and functions compared with NU. Elevations in intermediate monocytes were positively correlated with measures of cannabis use and BDI-II score.
Competing Interests: The authors declare there are no competing interests.
Databáze: MEDLINE
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