Altering Specificity and Autoactivity of Plant Immune Receptors Sr33 and Sr50 Via a Rational Engineering Approach.

Autor: Tamborski J; Department of Plant and Microbial Biology, University of California Berkeley, Berkeley, CA 94720, U.S.A., Seong K; Department of Plant and Microbial Biology, University of California Berkeley, Berkeley, CA 94720, U.S.A., Liu F; Department of Plant and Microbial Biology, University of California Berkeley, Berkeley, CA 94720, U.S.A.; Innovative Genomics Institute, University of California Berkeley, 2151 Berkeley Way, Berkeley, CA 94720, U.S.A., Staskawicz BJ; Department of Plant and Microbial Biology, University of California Berkeley, Berkeley, CA 94720, U.S.A.; Innovative Genomics Institute, University of California Berkeley, 2151 Berkeley Way, Berkeley, CA 94720, U.S.A., Krasileva KV; Department of Plant and Microbial Biology, University of California Berkeley, Berkeley, CA 94720, U.S.A.; Innovative Genomics Institute, University of California Berkeley, 2151 Berkeley Way, Berkeley, CA 94720, U.S.A.
Jazyk: angličtina
Zdroj: Molecular plant-microbe interactions : MPMI [Mol Plant Microbe Interact] 2023 Jul; Vol. 36 (7), pp. 434-446. Date of Electronic Publication: 2023 Aug 14.
DOI: 10.1094/MPMI-07-22-0154-R
Abstrakt: Many resistance genes deployed against pathogens in crops are intracellular nucleotide-binding (NB) leucine-rich repeat (LRR) receptors (NLRs). The ability to rationally engineer the specificity of NLRs will be crucial in the response to newly emerging crop diseases. Successful attempts to modify NLR recognition have been limited to untargeted approaches or depended on previously available structural information or knowledge of pathogen-effector targets. However, this information is not available for most NLR-effector pairs. Here, we demonstrate the precise prediction and subsequent transfer of residues involved in effector recognition between two closely related NLRs without their experimentally determined structure or detailed knowledge about their pathogen effector targets. By combining phylogenetics, allele diversity analysis, and structural modeling, we successfully predicted residues mediating interaction of Sr50 with its cognate effector AvrSr50 and transferred recognition specificity of Sr50 to the closely related NLR Sr33. We created synthetic versions of Sr33 that contain amino acids from Sr50, including Sr33 syn , which gained the ability to recognize AvrSr50 with 12 amino-acid substitutions. Furthermore, we discovered that sites in the LRR domain needed to transfer recognition specificity to Sr33 also influence autoactivity in Sr50. Structural modeling suggests these residues interact with a part of the NB-ARC domain, which we named the NB-ARC latch, to possibly maintain the inactive state of the receptor. Our approach demonstrates rational modifications of NLRs, which could be useful to enhance existing elite crop germplasm. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license.
Competing Interests: Competing interests: K. V. Krasileva, B. J. Staskawicz, and F. Liu have previously filed a patent application related to this work (patent publication number: WO/2021/113569; publication date: October 6, 2021; international application number: PCT/US2020/063203; international filing date: April 12, 2020).
Databáze: MEDLINE
Externí odkaz: