Photodynamic therapy reduces cell viability, migration and triggers necroptosis in prostate tumor cells.

Autor: de Melo Gomes LC; Department of Cell Biology, Histology and Embryology. Institute of Biomedical Sciences-ICBIM, Federal University of Uberlândia-UFU, Uberlândia, Minas Gerais, Brazil., de Oliveira Cunha AB; Department of Cell Biology, Histology and Embryology. Institute of Biomedical Sciences-ICBIM, Federal University of Uberlândia-UFU, Uberlândia, Minas Gerais, Brazil., Peixoto LFF; Department of Cell Biology, Histology and Embryology. Institute of Biomedical Sciences-ICBIM, Federal University of Uberlândia-UFU, Uberlândia, Minas Gerais, Brazil., Zanon RG; Department of Anatomy. Institute of Biomedical Sciences-ICBIM, Federal University of Uberlândia-UFU, Uberlândia, Minas Gerais, Brazil., Botelho FV; Institute of Biotechnology-IBTEC, Federal University of Uberlândia-UFU, Uberlândia, Minas Gerais, Brazil., Silva MJB; Department of Immunology, Institute of Biomedical Sciences-ICBIM, Federal University of Uberlândia-UFU, Uberlândia, Minas Gerais, Brazil., Pinto-Fochi ME; Faculdade de Medicina, União das Faculdades Dos Grandes Lagos, São José Do Rio Preto-São Paulo, São Paulo, Brazil., Góes RM; Department of Biology, Institute of Biosciences, Humanities and Exact Sciences, São Paulo State University-UNESP, São José Do Rio Preto-São Paulo, Brazil., de Paoli F; Department of Morphology, Institute of Biological Sciences, Federal University of Juiz de Fora-UFJF, Juiz de Fora, Minas Gerais, Brazil., Ribeiro DL; Department of Cell Biology, Histology and Embryology. Institute of Biomedical Sciences-ICBIM, Federal University of Uberlândia-UFU, Uberlândia, Minas Gerais, Brazil. daniele.ribeiro@ufu.br.
Jazyk: angličtina
Zdroj: Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology [Photochem Photobiol Sci] 2023 Jun; Vol. 22 (6), pp. 1341-1356. Date of Electronic Publication: 2023 Mar 03.
DOI: 10.1007/s43630-023-00382-9
Abstrakt: Prostate cancer is the most common cancer in American men, aside from skin cancer. As an alternative cancer treatment, photodynamic laser therapy (PDT) can be used to induce cell death. We evaluated the PDT effect, using methylene blue as a photosensitizer, in human prostate tumor cells (PC3). PC3 were subjected to four different conditions: DMEM (control); laser treatment (L-660 nm, 100 mW, 100 J.cm -2 ); methylene blue treatment (MB-25 μM, 30 min), and MB treatment followed by low-level red laser irradiation (MB-PDT). Groups were evaluated after 24 h. MB-PDT treatment reduced cell viability and migration. However, because MB-PDT did not significantly increase the levels of active caspase-3 and BCL-2, apoptosis was not the primary mode of cell death. MB-PDT, on the other hand, increased the acid compartment by 100% and the LC3 immunofluorescence (an autophagy marker) by 254%. Active MLKL level, a necroptosis marker, was higher in PC3 cells after MB-PDT treatment. Furthermore, MB-PDT resulted in oxidative stress due to a decrease in total antioxidant potential, catalase levels, and increased lipid peroxidation. According to these findings, MB-PDT therapy is effective at inducing oxidative stress and reducing PC3 cell viability. In such therapy, necroptosis is also an important mechanism of cell death triggered by autophagy.
(© 2023. The Author(s), under exclusive licence to European Photochemistry Association, European Society for Photobiology.)
Databáze: MEDLINE
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