[Pleiomorphism of the cytokine profile in nasal polyp tissue depending on the phenotype of chronic rhinosinusitis with nasal polyps].

Autor:	Savlevich EL; Central State Medical Academy of Administrative Directorate of the President of the Russian Federation, Moscow, Russia., Zurochka AV; Institute of Immunology and Physiology of the Ural Branch of the Russian Academy of Sciences, Yekaterinburg, Russia.; South Ural State University (National Research University), Chelyabinsk, Russia., Kurbacheva OM; Institute of Immunology, Moscow, Russia., Egorov VI; Vladimirsky Moscow Regional Research Clinical Institute, Moscow, Russia., Shilovskiy IP; Institute of Immunology, Moscow, Russia., Mitrofanova ES; Federal Scientific and Clinical Center for Specialized Types of Medical Care and Medical Technologies, Moscow, Russia., Lyubimova EV; ENT Clinic LLC, Yekaterinburg, Russia.
Jazyk:	ruština
Zdroj:	Vestnik otorinolaringologii [Vestn Otorinolaringol] 2023; Vol. 88 (1), pp. 50-56.
DOI:	10.17116/otorino20228801150
Abstrakt:	Clinical phenotypes of chronic rhinosinusitis with nasal polyps (CRSwNP) are characterized with different inflammation patterns of mRNA expression of cytokines and depend on presence of allergic rhinitis (AR), atopic bronchial asthma (aBA) or nonatopic bronchial asthma (nBA). Objective: To compare inflammation response in patients with different phenotypes of CRSwNP according to level secretion of the key cytokines in nasal polyp tissue. Material and Methods: 292 patients with CRSwNP were divided into four phenotypes: group 1 - CRSwNP without respiratory allergy (RA) and without BA; group 2a - CRSwNP+ AR with aBA; group 2b - CRSwNP+AR without aBA; group 3 - CRSwNP+nBA. Control group ( n =36) included patients with hypertrophic rhinitis without atopy or BA. Using multiplex assay we defined the level of IL-1β, IL-4, IL-5, IL-6, IL-13, IFN-γ, TGF-β1, TGF-β2, TGF-β3 in nasal polyp tissue. Results: The evaluation of cytokines levels in nasal polyps in different CRSwNP phenotypes showed a pleiotropy of different cytokine secretion depending on different comorbid pathology. In control group we estimated the lowest levels of all detected cytokines in comparison with other CRS groups. High levels of local proteins IL-5 and IL-13 and low levels of all isoform of TGF-β characterized CRSwNP without RA and BA. The combination of CRSwNP with AR showed high levels of proinflammatory cytokines IL-6 and IL-1β, and high levels of TGF-β1 and TGF-β2. The combination of CRSwNP with aBA estimated low levels of proinflammatory cytokines IL-1β, IFN-γ; in case of CRS+nBA we determined the highest levels of TGF-β1, TGF-β2 and TGF-β3 in nasal polyp tissue. Conclusions: Each CRSwNP phenotype is characterized by different mechanism of local inflammation. This underlies the necessity to diagnose BA and respiratory allergy among these patients. The evaluation of local cytokine profile in different CRSwNP phenotypes can help to determine the target anticytokine therapy for patients who has low efficacy of basic corticosteroid therapy.
Databáze:	MEDLINE
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