Differential CD4+ T-Cell Cytokine and Cytotoxic Responses Between Reactivation and Latent Phases of Herpes Zoster Infection.

Autor:	Jin W; Division of Infectious Diseases Case Western Reserve University, Cleveland, OH.; Division of Infectious Diseases Case Western Reserve University, Cleveland, OH., Fang M; Department of Population and Quantitative Health Sciences, Case Western Reserve University, Cleveland, OH., Sayin I; Division of Infectious Diseases Case Western Reserve University, Cleveland, OH.; Division of Infectious Diseases and Geriatric Research, Education and Clinical Center (GRECC), Cleveland Veterans Administration Medical Center, Cleveland, OH., Smith C; Division of Infectious Diseases Case Western Reserve University, Cleveland, OH.; Division of Infectious Diseases and Geriatric Research, Education and Clinical Center (GRECC), Cleveland Veterans Administration Medical Center, Cleveland, OH., Hunter JL; Department of Family Medicine, Ohio University, Athens, OH., Richardson B; Department of Population and Quantitative Health Sciences, Case Western Reserve University, Cleveland, OH., Golden JB; Department of Population and Quantitative Health Sciences, Case Western Reserve University, Cleveland, OH., Haley C; Center for Clinical Studies and Department of Dermatology, University of Texas Health Science Center, McGovern School of Medicine, Houston, TX., Schmader KE; Division of Geriatrics, Duke University Medical Center and GRECC, Durham Veterans Affairs Medical Center, Durham, NC., Betts MR; Department of Microbiology, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA., Tyring SK; Center for Clinical Studies and Department of Dermatology, University of Texas Health Science Center, McGovern School of Medicine, Houston, TX., Cameron CM; Department of Nutrition, Case Western Reserve University, Cleveland, OH., Cameron MJ; Department of Population and Quantitative Health Sciences, Case Western Reserve University, Cleveland, OH., Canaday DH; Division of Infectious Diseases Case Western Reserve University, Cleveland, OH.; Division of Infectious Diseases and Geriatric Research, Education and Clinical Center (GRECC), Cleveland Veterans Administration Medical Center, Cleveland, OH.
Jazyk:	angličtina
Zdroj:	Pathogens & immunity [Pathog Immun] 2023 Feb 17; Vol. 7 (2), pp. 171-188. Date of Electronic Publication: 2023 Feb 17 (Print Publication: 2022).
DOI:	10.20411/pai.v7i2.560
Abstrakt:	Background: CD4+ T cells are a critical component of effective immune responses to varicella zoster virus (VZV), but their functional properties during the reactivation acute vs latent phase of infection remain poorly defined. Methods: Here we assessed the functional and transcriptomic properties of peripheral blood CD4+ T cells in persons with acute herpes zoster (HZ) compared to those with a prior history of HZ infection using multicolor flow cytometry and RNA sequencing. Results: We found significant differences between the polyfunctionality of VZV-specific total memory, effector memory, and central memory CD4+ T cells in acute vs prior HZ. VZV-specific CD4+ memory T-cell responses in acute HZ reactivation had higher frequencies of IFN-γ and IL-2 producing cells compared to those with prior HZ. In addition, cytotoxic markers were higher in VZV-specific CD4+ T cells than non-VZV-specific cells. Transcriptomic analysis of ex vivo total memory CD4+ T cells from these individuals showed differential regulation of T-cell survival and differentiation pathways, including TCR, cytotoxic T lymphocytes (CTL), T helper, inflammation, and MTOR signaling pathways. These gene signatures correlated with the frequency of IFN-γ and IL-2 producing cells responding to VZV. Conclusions: In summary, VZV-specific CD4+ T cells from acute HZ individuals had unique functional and transcriptomic features, and VZV-specific CD4+ T cells as a group had a higher expression of cytotoxic molecules including Perforin, Granzyme-B, and CD107a. Competing Interests: The authors have declared that no competing interests exist. (Copyright © 2023 Pathogens and Immunity.)
Databáze:	MEDLINE
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