A bacterial sulfoglycosidase highlights mucin O-glycan breakdown in the gut ecosystem.

Autor: Katoh T; Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, Japan., Yamada C; Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan., Wallace MD; School of Molecular Sciences, The University of Western Australia, Crawley, WA, Australia., Yoshida A; Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan., Gotoh A; Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, Japan., Arai M; Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, Japan., Maeshibu T; Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, Japan., Kashima T; Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan., Hagenbeek A; Laboratory of Systems and Synthetic Biology, Wageningen University & Research, Wageningen, The Netherlands., Ojima MN; Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, Japan., Takada H; Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, Japan., Sakanaka M; Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, Japan., Shimizu H; Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, Japan., Nishiyama K; Department of Microbiology, School of Pharmacy, Kitasato University, Minato-ku, Tokyo, Japan., Ashida H; Faculty of Biology-Oriented Science and Technology, Kindai University, Kinokawa, Wakayama, Japan., Hirose J; School of Human Cultures, The University of Shiga Prefecture, Hikone, Shiga, Japan.; Department of Food and Nutrition, Kyoto Women's University, Kyoto, Japan., Suarez-Diez M; Laboratory of Systems and Synthetic Biology, Wageningen University & Research, Wageningen, The Netherlands., Nishiyama M; Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan., Kimura I; Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, Japan., Stubbs KA; School of Molecular Sciences, The University of Western Australia, Crawley, WA, Australia., Fushinobu S; Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo, Japan. asfushi@mail.ecc.u-tokyo.ac.jp., Katayama T; Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto, Japan. takane@lif.kyoto-u.ac.jp.
Jazyk: angličtina
Zdroj: Nature chemical biology [Nat Chem Biol] 2023 Jun; Vol. 19 (6), pp. 778-789. Date of Electronic Publication: 2023 Mar 02.
DOI: 10.1038/s41589-023-01272-y
Abstrakt: Mucinolytic bacteria modulate host-microbiota symbiosis and dysbiosis through their ability to degrade mucin O-glycans. However, how and to what extent bacterial enzymes are involved in the breakdown process remains poorly understood. Here we focus on a glycoside hydrolase family 20 sulfoglycosidase (BbhII) from Bifidobacterium bifidum, which releases N-acetylglucosamine-6-sulfate from sulfated mucins. Glycomic analysis showed that, in addition to sulfatases, sulfoglycosidases are involved in mucin O-glycan breakdown in vivo and that the released N-acetylglucosamine-6-sulfate potentially affects gut microbial metabolism, both of which were also supported by a metagenomic data mining analysis. Enzymatic and structural analysis of BbhII reveals the architecture underlying its specificity and the presence of a GlcNAc-6S-specific carbohydrate-binding module (CBM) 32 with a distinct sugar recognition mode that B. bifidum takes advantage of to degrade mucin O-glycans. Comparative analysis of the genomes of prominent mucinolytic bacteria also highlights a CBM-dependent O-glycan breakdown strategy used by B. bifidum.
(© 2023. The Author(s), under exclusive licence to Springer Nature America, Inc.)
Databáze: MEDLINE
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