Breeding and Reproduction of the African Turquoise Killifish Nothobranchius furzeri .
Autor: | Chen J; Department of Genetics, Stanford University, Stanford, California 94305, USA., Khondker RC; Department of Genetics, Stanford University, Stanford, California 94305, USA., Brunet A; Department of Genetics, Stanford University, Stanford, California 94305, USA abrunet1@stanford.edu.; Glenn Laboratories for the Biology of Aging, Stanford University, Stanford, California 94305, USA. |
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Jazyk: | angličtina |
Zdroj: | Cold Spring Harbor protocols [Cold Spring Harb Protoc] 2023 Jun 01; Vol. 2023 (6), pp. pdb.prot107816. Date of Electronic Publication: 2023 Jun 01. |
DOI: | 10.1101/pdb.prot107816 |
Abstrakt: | The successful breeding and reproduction of the African turquoise killifish Nothobranchius furzeri in a controlled laboratory setting are required to establish this fish species as a model system for studying vertebrate development and aging. Here, we describe a protocol to care for and hatch African turquoise killifish embryos, raise the juvenile fish to adulthood, and breed this species using sand as the breeding bedding. We also provide suggestions for generating a large quantity of good-quality embryos. (© 2023 Cold Spring Harbor Laboratory Press.) |
Databáze: | MEDLINE |
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