Ultrafast Energy Transfer from Photoexcited Tryptophan to the Haem in Cytochrome c.

Autor: Bacellar C; Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratoire de Spectroscopie Ultrarapide (LSU), ISIC and Lausanne Centre for Ultrafast Science (LACUS), CH-1015 Lausanne, Switzerland.; SwissFEL, Paul-Scherrer-Institut (PSI), 5232 Villigen PSI, Switzerland., Rouxel JR; Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratoire de Spectroscopie Ultrarapide (LSU), ISIC and Lausanne Centre for Ultrafast Science (LACUS), CH-1015 Lausanne, Switzerland.; Univ Lyon, UJM-Saint-Etienne, CNRS, Graduate School Optics Institute, Laboratoire Hubert Curien, UMR 5516, Saint-Etienne F-42023, France., Ingle RA; Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratoire de Spectroscopie Ultrarapide (LSU), ISIC and Lausanne Centre for Ultrafast Science (LACUS), CH-1015 Lausanne, Switzerland.; Department of Chemistry, University College London, 20 Gordon Street, London WC1H 0AJ, United Kingdom., Mancini GF; Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratoire de Spectroscopie Ultrarapide (LSU), ISIC and Lausanne Centre for Ultrafast Science (LACUS), CH-1015 Lausanne, Switzerland.; 2Laboratory for Ultrafast X-ray and Electron Microscopy, Department of Physics, University of Pavia, Via Agostino Bassi 6, 27100 Pavia PV, Italy., Kinschel D; Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratoire de Spectroscopie Ultrarapide (LSU), ISIC and Lausanne Centre for Ultrafast Science (LACUS), CH-1015 Lausanne, Switzerland., Cannelli O; Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratoire de Spectroscopie Ultrarapide (LSU), ISIC and Lausanne Centre for Ultrafast Science (LACUS), CH-1015 Lausanne, Switzerland., Zhao Y; Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratoire de Spectroscopie Ultrarapide (LSU), ISIC and Lausanne Centre for Ultrafast Science (LACUS), CH-1015 Lausanne, Switzerland., Cirelli C; SwissFEL, Paul-Scherrer-Institut (PSI), 5232 Villigen PSI, Switzerland., Knopp G; SwissFEL, Paul-Scherrer-Institut (PSI), 5232 Villigen PSI, Switzerland., Szlachetko J; SOLARIS National Synchrotron Radiation Centre, Jagiellonian University, 30-392 Kraków, Poland., Lima FA; European XFEL, Holzkoppel 4, D-22869 Schenefeld, Germany., Menzi S; SwissFEL, Paul-Scherrer-Institut (PSI), 5232 Villigen PSI, Switzerland., Ozerov D; SwissFEL, Paul-Scherrer-Institut (PSI), 5232 Villigen PSI, Switzerland., Pamfilidis G; SwissFEL, Paul-Scherrer-Institut (PSI), 5232 Villigen PSI, Switzerland., Kubicek K; European XFEL, Holzkoppel 4, D-22869 Schenefeld, Germany., Khakhulin D; European XFEL, Holzkoppel 4, D-22869 Schenefeld, Germany., Gawelda W; European XFEL, Holzkoppel 4, D-22869 Schenefeld, Germany.; Faculty of Physics, Adam Mickiewicz University, ul. Uniwersytetu Poznanskiego 2, 61-614 Poznan, Poland.; Departamento de Química, Universidad Autónoma de Madrid, 28049 Madrid, Spain.; IMDEA Nanociencia, Calle Faraday 9, 28049 Madrid, Spain., Rodriguez-Fernandez A; European XFEL, Holzkoppel 4, D-22869 Schenefeld, Germany., Biednov M; European XFEL, Holzkoppel 4, D-22869 Schenefeld, Germany., Bressler C; European XFEL, Holzkoppel 4, D-22869 Schenefeld, Germany., Arrell CA; SwissFEL, Paul-Scherrer-Institut (PSI), 5232 Villigen PSI, Switzerland., Johnson PJM; SwissFEL, Paul-Scherrer-Institut (PSI), 5232 Villigen PSI, Switzerland., Milne CJ; SwissFEL, Paul-Scherrer-Institut (PSI), 5232 Villigen PSI, Switzerland.; European XFEL, Holzkoppel 4, D-22869 Schenefeld, Germany., Chergui M; Ecole Polytechnique Fédérale de Lausanne (EPFL), Laboratoire de Spectroscopie Ultrarapide (LSU), ISIC and Lausanne Centre for Ultrafast Science (LACUS), CH-1015 Lausanne, Switzerland.
Jazyk: angličtina
Zdroj: The journal of physical chemistry letters [J Phys Chem Lett] 2023 Mar 09; Vol. 14 (9), pp. 2425-2432. Date of Electronic Publication: 2023 Mar 02.
DOI: 10.1021/acs.jpclett.3c00218
Abstrakt: We report femtosecond Fe K-edge absorption (XAS) and nonresonant X-ray emission (XES) spectra of ferric cytochrome C (Cyt c) upon excitation of the haem (>300 nm) or mixed excitation of the haem and tryptophan (<300 nm). The XAS and XES transients obtained in both excitation energy ranges show no evidence for electron transfer processes between photoexcited tryptophan (Trp) and the haem, but rather an ultrafast energy transfer, in agreement with previous ultrafast optical fluorescence and transient absorption studies. The reported ( J. Phys. Chem. B 2011 , 115 (46), 13723-13730) decay times of Trp fluorescence in ferrous (∼350 fs) and ferric (∼700 fs) Cyt c are among the shortest ever reported for Trp in a protein. The observed time scales cannot be rationalized in terms of Förster or Dexter energy transfer mechanisms and call for a more thorough theoretical investigation.
Databáze: MEDLINE