Quantify permeability using on-a-chip models in high-throughput applications.

Autor:	Soragni C; MIMETAS BV, De Limes 7, 2342 DH Oegstgeest, the Netherlands; Department of Cardiology, Maastricht University, P. Debyelaan 25, 6229 HX Maastricht, the Netherlands. Electronic address: c.soragni@maastrichtuniversity.nl., Vergroesen T; MIMETAS BV, De Limes 7, 2342 DH Oegstgeest, the Netherlands., Hettema N; MIMETAS BV, De Limes 7, 2342 DH Oegstgeest, the Netherlands., Rabussier G; MIMETAS BV, De Limes 7, 2342 DH Oegstgeest, the Netherlands; Department of Cardiology, Maastricht University, P. Debyelaan 25, 6229 HX Maastricht, the Netherlands., Lanz HL; MIMETAS BV, De Limes 7, 2342 DH Oegstgeest, the Netherlands., Trietsch SJ; MIMETAS BV, De Limes 7, 2342 DH Oegstgeest, the Netherlands., de Windt LJ; Department of Cardiology, Maastricht University, P. Debyelaan 25, 6229 HX Maastricht, the Netherlands., Ng CP; MIMETAS BV, De Limes 7, 2342 DH Oegstgeest, the Netherlands. Electronic address: c.ng@mimetas.com.
Jazyk:	angličtina
Zdroj:	STAR protocols [STAR Protoc] 2023 Mar 17; Vol. 4 (1), pp. 102051. Date of Electronic Publication: 2023 Jan 20.
DOI:	10.1016/j.xpro.2023.102051
Abstrakt:	Traditionally, to quantify permeability of a biological barrier, the initial slope is used, based on the assumption of sink condition (concentration of the donor is constant, and the receiver increases less than 10%). With on-a-chip barrier models, this assumption fails in cell-free or leaky conditions, which requires the use of the exact solution. To encounter a time delay from performing the assay and acquiring the data, we present a protocol with the exact equation modified to incorporate a time offset. Competing Interests: Declaration of interests C.S., T.V., and N.H. were employed by MIMETAS BV when the data were generated; G.R., H.L.L., S.J.T., and C.P.N. are employees of Mimetas BV, which is marketing the OrganoPlate; and S.J.T. is a shareholder of Mimetas BV. OrganoPlate is a registered trademark of Mimetas BV. (Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.)
Databáze:	MEDLINE
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