Group B streptococcus virulence factors associated with different clinical syndromes: Asymptomatic carriage in pregnant women and early-onset disease in the newborn.

Autor:	Schindler Y; Laboratory of Microbiology, Mayanei Hayeshua Medical Center, Bnei Brak, Israel.; Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel., Rahav G; Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.; Infectious Disease Unit, Sheba Medical Center, Tel HaShomer, Israel., Nissan I; Infectious Disease Unit, Sheba Medical Center, Tel HaShomer, Israel.; National Public Health Laboratory, Ministry of Health, Tel Aviv, Israel., Treygerman O; Laboratory of Microbiology, Meuhedet Health Maintenance Organization, Lod, Israel., Prajgrod G; Laboratory of Microbiology, Meuhedet Health Maintenance Organization, Lod, Israel., Attia BZ; Laboratory of Microbiology, Mayanei Hayeshua Medical Center, Bnei Brak, Israel., Raz R; Laboratory of Microbiology, Mayanei Hayeshua Medical Center, Bnei Brak, Israel., Valenci GZ; National Public Health Laboratory, Ministry of Health, Tel Aviv, Israel., Tekes-Manova D; Laboratory of Microbiology, Mayanei Hayeshua Medical Center, Bnei Brak, Israel., Maor Y; Sackler School of Medicine, Tel Aviv University, Tel Aviv, Israel.; Infectious Disease Unit, Wolfson Medical Center, Holon, Israel.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2023 Feb 13; Vol. 14, pp. 1093288. Date of Electronic Publication: 2023 Feb 13 (Print Publication: 2023).
DOI:	10.3389/fmicb.2023.1093288
Abstrakt:	Background: Group B streptococcus (GBS) harbors many virulence factors but there is limited data regarding their importance in colonization in pregnancy and early-onset disease (EOD) in the newborn. We hypothesized that colonization and EOD are associated with different distribution and expression of virulence factors. Methods: We studied 36 GBS EOD and 234 GBS isolates collected during routine screening. Virulence genes (pilus-like structures- PI-1, PI-2a, PI-2b ; rib and hvgA ) presence and expression were identified by PCR and qRT-PCR. Whole genome sequencing (WGS) and comparative genomic analyses were used to compare coding sequences (CDSs) of colonizing and EOD isolates. Results: Serotype III (ST17) was significantly associated with EOD and serotype VI (ST1) with colonization. hvgA and rib genes were more prevalent among EOD isolates (58.3 and 77.8%, respectively; p < 0.01). The pilus loci PI-2b and PI-2a were more prevalent among EOD isolates (61.1%, p < 0.01), while the pilus loci PI-2a and PI-1 among colonizing isolates (89.7 and 93.1% vs. 55.6 and 69.4%, p < 0.01). qRT PCR analysis revealed that hvgA was barely expressed in colonizing isolates, even though the gene was detected. Expression of the rib gene and PI-2b was two-fold higher in EOD isolates compared to colonizing isolates. Transcription of PI-2a was three-fold higher in colonizing isolates compared to EOD isolates. ST17 isolates (associated with EOD) had a smaller genome size compared ST1 and the genome was more conserved relative to the reference strain and ST17 isolates. In a multivariate logistic regression analysis virulence factors independently associated with EOD were serotype 3, and PI-1 and PI-2a was protective. Conclusion: There was a significant difference in the distribution of hvg A , rib , and PI genes among EOD (serotype III/ST17) and colonizing (serotype VI/ST1) isolates suggesting an association between invasive disease and these virulence factors. Further study is needed to understand the contribution of these genes to GBS virulence. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Schindler, Rahav, Nissan, Treygerman, Prajgrod, Attia, Raz, Valenci, Tekes-Manova and Maor.)
Databáze:	MEDLINE
Externí odkaz:	Zobrazit plný text záznamu