Effects of Dietary Pectin and Lactobacillus salivarius ATCC 11741 on Growth Performance, Immunocompetence, Gut Microbiota, Antioxidant Capacity, and Disease Resistance in Narrow-Clawed Crayfish, Postantacus leptodactylus .

Autor: Jastaniah SDS; Biological Sciences Department, Faculty of Sciences, King Abdulaziz University, Jeddah, Saudi Arabia., Hafsan H; Biology Department, Universitas Islam Negeri Alauddin, Indonesia., Tseng CJ; Assistant Professor, Rattanakosin International College of Creative Entrepreneurship, Rajamangala University of Technology Rattanakosin, Thailand., Karim YS; Department of Pharmacy, Al-Manara College for Medical Sciences, Maysan, Iraq., Hamza MU; Medical Technical College, Al-Farahidi University, Iraq., Hameed NM; Anesthesia Techniques, Al-Nisour University College, Iraq., Al-Zubaidi SH; Anesthesia Techniques Department, Al-Mustaqbal University College, Babylon, Iraq., Almotlaq SSK; Microbiology Research Group, College of Pharmacy, Al-Ayen University, Thi-Qar, Iraq., Yasin G; Department of Botany, Bahauddin Zakariya University, Multan, Pakistan., Iswanto AH; Public Health Department, Faculty of Health Science, University of Pembangunan Nasional Veteran Jakarta, Jakarta, Indonesia., Dadras M; Department of Fisheries, Faculty of Natural Resources, University of Tehran, Karaj, Iran., Chorehi MM; Department of Fisheries, Faculty of Natural Resources, Guilan University, Rasht, Iran.
Jazyk: angličtina
Zdroj: Aquaculture nutrition [Aquac Nutr] 2022 Nov 02; Vol. 2022, pp. 1861761. Date of Electronic Publication: 2022 Nov 02 (Print Publication: 2022).
DOI: 10.1155/2022/1861761
Abstrakt: The present study was conducted to clarify the effects of Lactobacillus salivarius (LS) ATCC 11741 and pectin (PE) on growth performance, digestive enzymes activity, gut microbiota composition, immune parameters, antioxidant defense as well as disease resistance against Aeromonas hydrophila in narrow-clawed crayfish, Postantacus leptodactylus . During 18 weeks trial feeding, 525 narrow-clawed crayfish juvenile (8.07 ± 0.1 g) fed with seven experimental diets including control (basal diet), LS1 (1 × 10 7  CFU/g), LS2 (1 × 109 CFU/g), PE1 (5 g/kg), PE2 (10 g/kg), LS1PE1 (1 × 10 7  CFU/g +5 g/kg), and LS2PE2 (1 × 109 CFU/g +10 g/kg). After 18 weeks, growth parameters (final weight, weight gain, and specific growth rate) and feed conversion rate were significantly improved in all treatments ( P < 0.05). Besides, diets incorporated with LS1PE1 and LS2PE2 significantly increased the activity of amylase and protease enzymes compared to LS1, LS2, and control groups ( P < 0.05). Microbiological analyses revealed that the total heterotrophic bacteria count (TVC) and lactic acid bacteria (LAB) of narrow-clawed crayfish fed diets containing LS1, LS2, LS1PE1, and LS2PE2 were higher than control group. The highest total haemocyte count (THC), large-granular (LGC) and semigranular cells (SGC) count, and hyaline count (HC) was obtained in LS1PE1 ( P < 0.05). Similarly, higher immunity activity (lysozyme (LYZ), phenoloxidase (PO), nitroxidesynthetase (NOs), and alkaline phosphatase (AKP)) observed in the LS1PE1 treatment compared to the control group ( P < 0.05). The glutathione peroxidase (GPx) and superoxide dismutase (SOD) activity remarkably enhanced in LS1PE1 and LS2PE2, while malondialdehyde (MDA) content reduced in these two treatments. In addition, specimens belonging to LS1, LS2, PE2, LS1PE1, and LS2PE2 groups presented higher resistance against A. hydrophila compared to the control group. In conclusion, feeding narrow-clawed crayfish with synbiotic had higher efficiency on growth parameters, immunocompetence, and disease resistance compared to single consumption of prebiotics and probiotics.
Competing Interests: The authors have no conflicts of interest to declare.
(Copyright © 2022 Samyah Darwish Saddig Jastaniah et al.)
Databáze: MEDLINE
Nepřihlášeným uživatelům se plný text nezobrazuje