Effect of liposomal formulation of ascorbic acid on corneal permeability.

Autor: Csorba A; Department of Ophthalmology, Semmelweis University, Mária Street 39., 1085, Budapest, Hungary., Katona G; Institute of Pharmaceutical Technology and Regulatory Affairs, Faculty of Pharmacy, University of Szeged, Eötvös Street 6., 6720, Szeged, Hungary., Budai-Szűcs M; Institute of Pharmaceutical Technology and Regulatory Affairs, Faculty of Pharmacy, University of Szeged, Eötvös Street 6., 6720, Szeged, Hungary., Balogh-Weiser D; Department of Physical Chemistry and Materials Science, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem Rkp. 3., 1111, Budapest, Hungary., Fadda AM; Department of Life and Environmental Sciences, University of Cagliari, Via Ospedale 72, 09124, Cagliari, Italy., Caddeo C; Department of Life and Environmental Sciences, University of Cagliari, Via Ospedale 72, 09124, Cagliari, Italy., Takács ÁI; Department of Ophthalmology, Semmelweis University, Mária Street 39., 1085, Budapest, Hungary., Mátyus P; E-Group ICT SOFTWARE, Kacsa Street 11, 1027, Budapest, Hungary., Balogh GT; Department of Chemical and Process Engineering, Faculty of Chemical Technology and Biotechnology, Budapest University of Technology and Economics, Műegyetem Rkp. 3., 1111, Budapest, Hungary. balogh.gyorgy@vbk.bme.hu.; Institute of Pharmacodynamics and Biopharmacy, Faculty of Pharmacy, University of Szeged, Eötvös Street 6., 6720, Szeged, Hungary. balogh.gyorgy@vbk.bme.hu., Nagy ZZ; Department of Ophthalmology, Semmelweis University, Mária Street 39., 1085, Budapest, Hungary. nagy.zoltan_zsolt@med.semmelweis-univ.hu.
Jazyk: angličtina
Zdroj: Scientific reports [Sci Rep] 2023 Mar 01; Vol. 13 (1), pp. 3448. Date of Electronic Publication: 2023 Mar 01.
DOI: 10.1038/s41598-023-29290-9
Abstrakt: Ascorbic acid (AA) has a pivotal role in corneal wound healing via stimulating the biosynthesis of highly organized extracellular matrix components, but its rapid degradation and low corneal permeability limits its therapeutic effects. In this paper, we present the pharmacokinetic properties of a liposomal-based formulation of AA in terms of corneal permeation. Chemical stability, shelf-life, and drug release rate of lyophilized liposome (AA-LLipo) formulation was determined in comparison to free-form of AA solution using high-performance liquid chromatography (HPLC) and rapid equilibrium dialysis. In vitro transcorneal permeability was studied using a parallel artificial membrane permeability assay (PAMPA). Ex vivo permeation was examined on AA-LLipo-treated porcine cornea by determining the AA content on the ocular surface, in the cornea as well as in the aqueous humor using HPLC, and by Raman-mapping visualizing the AA-distribution. Our results showed that the liposomal formulation improved the chemical stability of AA, while drug release was observed with the same kinetic efficiency as from the free-form of AA solution. Both corneal-PAMPA and porcine corneal permeability studies showed that AA-LLipo markedly improved the corneal absorption kinetics of AA, thus, increasing the AA content in the cornea and aqueous humor. AA-LLipo formulation could potentially increase the bioavailability of AA in corneal tissues.
(© 2023. The Author(s).)
Databáze: MEDLINE
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