An antisense intragenic lncRNA SEAIRa mediates transcriptional and epigenetic repression of SERRATE in Arabidopsis .

Autor:	Chen W; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.; Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore.; Centre for BioImaging Sciences, National University of Singapore, Singapore 117557, Singapore., Zhu T; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China., Shi Y; State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/Collaborative Innovation Center for Modern Crop Production (CIC-MCP), Nanjing, Jiangsu 210095, China., Chen Y; Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore.; Centre for BioImaging Sciences, National University of Singapore, Singapore 117557, Singapore., Li WJ; Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore.; Centre for BioImaging Sciences, National University of Singapore, Singapore 117557, Singapore., Chan RJ; Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore.; Centre for BioImaging Sciences, National University of Singapore, Singapore 117557, Singapore., Chen D; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China., Zhang W; State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/Collaborative Innovation Center for Modern Crop Production (CIC-MCP), Nanjing, Jiangsu 210095, China., Yuan YA; Department of Biological Sciences, National University of Singapore, Singapore 117543, Singapore.; Centre for BioImaging Sciences, National University of Singapore, Singapore 117557, Singapore.; Temasek Life Sciences Laboratory, National University of Singapore, Singapore 117604, Singapore., Wang X; State Key Laboratory of Crop Genetics and Germplasm Enhancement, Cytogenetics Institute, Nanjing Agricultural University/Collaborative Innovation Center for Modern Crop Production (CIC-MCP), Nanjing, Jiangsu 210095, China., Sun B; State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, Nanjing 210023, China.
Jazyk:	angličtina
Zdroj:	Proceedings of the National Academy of Sciences of the United States of America [Proc Natl Acad Sci U S A] 2023 Mar 07; Vol. 120 (10), pp. e2216062120. Date of Electronic Publication: 2023 Mar 01.
DOI:	10.1073/pnas.2216062120
Abstrakt:	SERRATE (SE) is a core protein for microRNA (miRNA) biogenesis as well as for mRNA alternative splicing. Investigating the regulatory mechanism of SE expression is hence critical to understanding its detailed function in diverse biological processes. However, little about the control of SE expression has been clarified, especially through long noncoding RNA (lncRNA). Here, we identified an antisense intragenic lncRNA transcribed from the 3' end of SE , named SEAIRa. SEAIRa repressed SE expression, which in turn led to serrated leaves. SEAIRa recruited plant U-box proteins PUB25/26 with unreported RNA binding ability and a ubiquitin-like protein related to ubiquitin 1 (RUB1) for H2A monoubiquitination (H2Aub) at exon 11 of SE . In addition, PUB25/26 helped cleave SEAIRa and release the 5' domain fragment, which recruited the PRC2 complex for H3 lysine 27 trimethylation (H3K27me3) deposition at the first exon of SE . The distinct modifications of H2Aub and H3K27me3 at different sites of the SE locus cooperatively suppressed SE expression. Collectively, our results uncover an epigenetic mechanism mediated by the lncRNA SEAIRa that modulates SE expression, which is indispensable for plant growth and development.
Databáze:	MEDLINE
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