| Autor: |
Ali F; Dalian Medical University, Department of Cell Biology, Dalian, Liaoning, China., Ali S; Gulf Medical University, College of Health Sciences, Department of Medical Laboratory Sciences, Ajman, United Arab Emirates., Mohamed S; Gulf Medical University, College of Health Sciences, Department of Medical Laboratory Sciences, Ajman, United Arab Emirates., Khan I; Lanzhou University, School of Life Sciences, Department of Microbiology, Lanzhou, Gansu, China., Khan I; Khyber Medical University, Department of Microbiology, Peshawar, Pakistan., Khan S; Center for Reproductive Medicine, Henan Key Laboratory of Reproduction and Genetics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China., Khan F; Pir Mehr Ali Shah Arid Agriculture University, Department of Zoology, Rawalpindi, Pakistan., Alfeel AH; Gulf Medical University, College of Health Sciences, Department of Medical Laboratory Sciences, Ajman, United Arab Emirates., Higazi H; Gulf Medical University, College of Health Sciences, Department of Medical Laboratory Sciences, Ajman, United Arab Emirates. |
| Abstrakt: |
Heart and blood vessel disorders, such as coronary heart disease, brain vessel disease, rheumatic heart disease, and others, are together referred to as cardiovascular disease (CVD). In this study, we sought to determine how mitochondrial Leucine Transfer RNA genes and CVDs are related (MT-L1 and MT-L2). From CVD patients in Peshawar, a total of 27 saliva samples were taken. Leu-tRNA genes expressed by mitochondria were amplified using polymerase chain reaction after DNA was removed. Ten samples were sent for sequencing after PCR and gene cleaning. We obtained all of the sequenced results, which were subsequently aligned and evaluated against the mitochondrial revised Cambridge Reference Sequence (rCRS). However, in our sequenced samples, Leu-tRNA MT-L1 and MT-L2 genes were determined to be unaltered. Thus, it is suggested that a large population be taken into account while screening for mutations in the mitochondrial encoded Leu-tRNA MT-L1 and MT-L2 genes of cardiac patients in areas of Pakistan. Additionally, it is recommended that patients with cardiac problems should also have other mitochondrial encoded genes checked for potential mutations. This could result in the identification of genetic markers that could be used for early CVD screening in Pakistan. |
