Caffeine-tolerant mutations selected through an at-home yeast experimental evolution teaching lab.

Autor:	Moresi NG; Genome Sciences, University of Washington, Seattle, Washington, United States., Geck RC; Genome Sciences, University of Washington, Seattle, Washington, United States., Skophammer R; Westridge School, Pasadena, California, United States., Godin D; Genome Sciences, University of Washington, Seattle, Washington, United States., Students Y; Westridge School, Pasadena, California, United States., Taylor MB; Program in Biology, Loras College, Dubuque, Iowa, United States., Dunham MJ; Genome Sciences, University of Washington, Seattle, Washington, United States.
Jazyk:	angličtina
Zdroj:	MicroPublication biology [MicroPubl Biol] 2023 Feb 09; Vol. 2023. Date of Electronic Publication: 2023 Feb 09 (Print Publication: 2023).
DOI:	10.17912/micropub.biology.000749
Abstrakt:	yEvo is a curriculum for high school students centered around evolution experiments in S. cerevisiae . To adapt the curriculum for remote instruction, we created a new protocol to evolve non-engineered yeast in the presence of caffeine. Evolved strains had increased caffeine tolerance and distinct colony morphologies. Many possessed copy number variations, transposon insertions, and mutations affecting genes with known relationships to caffeine and TOR signaling - which is inhibited by caffeine - and in other genes not previously connected with caffeine. This demonstrates that our accessible, at-home protocol is sufficient to permit novel insights into caffeine tolerance. (Copyright: © 2023 by the authors.)
Databáze:	MEDLINE
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