Mesenchymal stem cell-derived neural progenitors attenuate proinflammatory microglial activation via paracrine mechanisms.

Autor:	Harris VK; Tisch Multiple Sclerosis Research Center of New York, NY 10019, USA., Bishop D; Tisch Multiple Sclerosis Research Center of New York, NY 10019, USA., Wollowitz J; Tisch Multiple Sclerosis Research Center of New York, NY 10019, USA., Carling G; Tisch Multiple Sclerosis Research Center of New York, NY 10019, USA., Carlson AL; Tisch Multiple Sclerosis Research Center of New York, NY 10019, USA., Daviaud N; Tisch Multiple Sclerosis Research Center of New York, NY 10019, USA., Sadiq SA; Tisch Multiple Sclerosis Research Center of New York, NY 10019, USA.
Jazyk:	angličtina
Zdroj:	Regenerative medicine [Regen Med] 2023 Mar; Vol. 18 (3), pp. 259-273. Date of Electronic Publication: 2023 Feb 27.
DOI:	10.2217/rme-2023-0005
Abstrakt:	Background: Mesenchymal stem cell-derived neural progenitor cell (MSC-NP) therapy is an experimental approach to treat multiple sclerosis. The influence of MSC-NPs on microglial activation was investigated. Methods: Microglia were stimulated in the presence of MSC-NP-conditioned media, and proinflammatory or proregenerative marker expression was assessed by quantitative PCR and ELISA. Results: Microglia stimulated in the presence of MSC-NP-conditioned media displayed reduced expression of proinflammatory markers including CCL2, increased expression of proregenerative markers and reduced phagocytic activity. The paracrine effects of MSC-NPs from multiple donors correlated with TGF-β3 gene expression and was reversed by TGF-β signaling inhibition. Conclusion: MSC-NPs promote beneficial microglial polarization through secreted factors. This study suggests that microglia are a potential therapeutic target of MSC-NP cell therapy.
Databáze:	MEDLINE
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