The Contribution of Multiplex Polymerase Chain Reaction in the Diagnosis of Central Nervous System Infections in Intensive Care Units.

Autor:	Debbagh F; Microbiology Laboratory, Faculty of Medicine and Pharmacy, Mohammed VI University Hospital of Marrakech, Cadi Ayyad University, Marrakech, MAR.; Biochemistry-Toxicology Laboratory, Avicenna Military Hospital, Marrakech, MAR., Harrar S; Microbiology Department, Arrazi Hospital, Mohammed VI University Hospital of Marrakech, Marrakech, MAR., Babokh F; Biology Department/Parasitology and Mycology Laboratory, Faculty of Medicine and Pharmacy, Mohammed VI University Hospital of Marrakech, Cadi Ayyad University, Marrakech, MAR., Lamrani Hanchi A; Microbiology Department, Arrazi Hospital, Mohammed VI University Hospital of Marrakech, Marrakech, MAR., Soraa N; Microbiology Laboratory, Arrazi Hospital, Mohammed VI University Hospital of Marrakech, Marrakech, MAR.
Jazyk:	angličtina
Zdroj:	Cureus [Cureus] 2023 Feb 22; Vol. 15 (2), pp. e35338. Date of Electronic Publication: 2023 Feb 22 (Print Publication: 2023).
DOI:	10.7759/cureus.35338
Abstrakt:	Introduction The aim of this study was to evaluate the contribution and limits of BioFire® FilmArray® meningitis/encephalitis panel (FA MEP) polymerase chain reaction (PCR) (bioMérieux, Marcy-l'Étoile, France) (product references: LLC RFIT-ASY-0118) coupled with bacterial and fungal culture in the diagnosis of central nervous system infections (CNSIs). Methods This was a retrospective observational study including all patients (adults and children) hospitalized in the intensive care units (ICUs) of a Moroccan university hospital, who benefited from multiplex PCR on a cerebrospinal fluid (CSF) sample. Results A total of 112 PCRs were performed, with a positivity rate of 18%. Bacterial etiology was the most frequent (70%), represented mainly by Streptococcus pneumoniae (45%), followed by viruses (25%), with four isolates of Herpes simplex virus (HSV) 1 . On 94 samples, there was an agreement between the culture and PCR results. Their discordance was found in 18 cases, including 16 suspected CNSIs recovered only by PCR and two diagnoses confirmed only by bacterial culture. Conclusion This study revealed the significant impact of multiplex PCR on the early and targeted diagnostic and therapeutic management of infectious meningitis and meningoencephalitis in intensive care unit patients. Competing Interests: The authors have declared that no competing interests exist. (Copyright © 2023, Debbagh et al.)
Databáze:	MEDLINE
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