| Autor: |
Avižinienė A; Institute of Biotechnology, Life Sciences Center, Vilnius University, LT-10257 Vilnius, Lithuania., Kučinskaitė-Kodzė I; Institute of Biotechnology, Life Sciences Center, Vilnius University, LT-10257 Vilnius, Lithuania., Petraitytė-Burneikienė R; Institute of Biotechnology, Life Sciences Center, Vilnius University, LT-10257 Vilnius, Lithuania., Žvirblienė A; Institute of Biotechnology, Life Sciences Center, Vilnius University, LT-10257 Vilnius, Lithuania., Mertens ML; Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut (FLI), Federal Research Institute for Animal Health, 17493 Greifswald-Insel Riems, Germany., Schmidt S; Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut (FLI), Federal Research Institute for Animal Health, 17493 Greifswald-Insel Riems, Germany.; German Society for Tissue Transplantation, Feodor-Lynen-Str. 21, 30625 Hannover, Germany., Schlegel M; Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut (FLI), Federal Research Institute for Animal Health, 17493 Greifswald-Insel Riems, Germany.; Seramun Diagnostica GmbH, Spreenhagener Street 1, 15754 Heidesee, Germany., Lattwein E; Institute for Experimental Immunology, Euroimmun Medical laboratory Diagnostics AG, Seekamp 31, 23560 Luebeck, Germany., Koellner B; Institute of Immunology, Friedrich-Loeffler-Institute, Federal Research Institute for Animal Health, 17493 Greifswald-Insel Riems, Germany., Ulrich RG; Institute of Novel and Emerging Infectious Diseases, Friedrich-Loeffler-Institut (FLI), Federal Research Institute for Animal Health, 17493 Greifswald-Insel Riems, Germany.; German Centre for Infection Research, Partner Site Hamburg-Lübeck-Borstel-Riems, Südufer 10, 17493 Greifswald-Insel Riems, Germany. |
| Abstrakt: |
Hantaviruses are emerging pathogens with a worldwide distribution that can cause life-threatening diseases in humans. Monoclonal antibodies (MAbs) against hantavirus nucleocapsid (N) proteins are important tools in virus diagnostics, epidemiological studies and basic research studies on virus replication and pathogenesis. Here, we extend the collection of previously generated MAbs raised against a segment of Puumala orthohantavirus (PUUV) N protein harbored on virus-like particles (VLPs) and MAbs against N proteins of Sin Nombre orthohantavirus/Andes orthohantavirus by generating nine novel MAbs against N proteins of Dobrava-Belgrade orthohantavirus (DOBV), Tula orthohantavirus (TULV), Thottapalayam thottimvirus (TPMV) and PUUV. In order to have a wide collection of well-described hantavirus-specific MAbs, the cross-reactivity of novel and previously generated MAbs was determined against N proteins of 15 rodent- and shrew-borne hantaviruses by different immunological methods. We found that all MAbs, excluding TPMV-specific MAbs, demonstrated different cross-reactivity patterns with N proteins of hantaviruses and recognized native viral antigens in infected mammalian cells. This well-characterized collection of cross-reactive hantavirus-specific MAbs has a potential application in various fields of hantavirus research, diagnostics and therapy. |
