| Autor: |
Vlasov ME; Federal Research Center for Virology and Microbiology (FRCVIM), Petushki District, Vladimir Region, 601125 Volginsky, Russia., Sindryakova IP; Federal Research Center for Virology and Microbiology (FRCVIM), Petushki District, Vladimir Region, 601125 Volginsky, Russia., Kudrjashov DA; Federal Research Center for Virology and Microbiology (FRCVIM), Petushki District, Vladimir Region, 601125 Volginsky, Russia., Morgunov SY; Federal Research Center for Virology and Microbiology (FRCVIM), Petushki District, Vladimir Region, 601125 Volginsky, Russia., Kolbasova OL; Federal Research Center for Virology and Microbiology (FRCVIM), Petushki District, Vladimir Region, 601125 Volginsky, Russia., Lyska VM; Federal Research Center for Virology and Microbiology (FRCVIM), Petushki District, Vladimir Region, 601125 Volginsky, Russia., Zhivoderov SP; Federal Research Center for Virology and Microbiology (FRCVIM), Petushki District, Vladimir Region, 601125 Volginsky, Russia., Pivova EY; Federal Research Center for Virology and Microbiology (FRCVIM), Petushki District, Vladimir Region, 601125 Volginsky, Russia., Balyshev VM; Federal Research Center for Virology and Microbiology (FRCVIM), Petushki District, Vladimir Region, 601125 Volginsky, Russia., Sereda AD; Federal Research Center for Virology and Microbiology (FRCVIM), Petushki District, Vladimir Region, 601125 Volginsky, Russia., Kolbasov DV; Federal Research Center for Virology and Microbiology (FRCVIM), Petushki District, Vladimir Region, 601125 Volginsky, Russia. |
| Abstrakt: |
African swine fever virus (ASFV) is an extremely genetically and phenotypically heterogeneous pathogen. Previously, we have demonstrated that experimental inoculation of pigs with an attenuated strain, Katanga-350 (genotype I, seroimmunotype I) (ASFV-Katanga-350), can induce protective immunity in 80% of European domestic pigs against the homologous virulent European strain Lisbon-57. At least 50% of the surviving pigs received protection from subsequent intramuscular infection with a heterologous virulent strain, Stavropol 01/08 (genotype II, seroimmunotype VIII) (ASFV-Stavropol 01/08). In this study, we assessed clinical signs, the levels of viremia, viral DNA, anti-ASFV antibodies and post-mortem changes caused by subsequent intramuscular injection with ASFV-Katanga-350 and heterologous ASFV-Stavropol 01/08. Inoculation of pigs with the ASFV-Katanga-350 did not protect animals from the disease in the case of the subsequent challenged ASFV-Stavropol 01/08. However, 40% of pigs were protected from death. Moreover, the surviving animals showed no pathomorphological changes or the presence of an infectious virus in the organs after euthanasia at 35 days post challenging. The ability/inability of attenuated strains to form a certain level of protection against heterologous isolates needs a theoretical background and experimental confirmation. |
