Detection of Hepatitis E Virus Genotype 3 in Feces of Capybaras (Hydrochoeris hydrochaeris) in Brazil.

Autor: Cunha L; Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil., Luchs A; Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil., Azevedo LS; Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil., Silva VCM; Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil., Lemos MF; Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil., Costa AC; Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo 05403-000, Brazil., Compri AP; Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil., França Y; Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil., Viana E; Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil., Malta F; Hospital Israelita Albert Einstein, Sao Paulo 05652-900, Brazil., Medeiros RS; Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil., Guiducci R; Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil., Morillo SG; Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil., Gomes-Gouvea MS; Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo 05403-000, Brazil., Amgarten D; Hospital Israelita Albert Einstein, Sao Paulo 05652-900, Brazil., Pinho JRR; Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo 05403-000, Brazil.; Hospital Israelita Albert Einstein, Sao Paulo 05652-900, Brazil., Moreira RC; Instituto Adolfo Lutz, Sao Paulo 01246-902, Brazil.
Jazyk: angličtina
Zdroj: Viruses [Viruses] 2023 Jan 24; Vol. 15 (2). Date of Electronic Publication: 2023 Jan 24.
DOI: 10.3390/v15020335
Abstrakt: Hepatitis E virus (HEV) is an emerging zoonotic pathogen associated with relevant public health issues. The aim of this study was to investigate HEV presence in free-living capybaras inhabiting urban parks in São Paulo state, Brazil. Molecular characterization of HEV positive samples was undertaken to elucidate the genetic diversity of the virus in these animals. A total of 337 fecal samples were screened for HEV using RT-qPCR and further confirmed by conventional nested RT-PCR. HEV genotype and subtype were determined using Sanger and next-generation sequencing. HEV was detected in one specimen (0.3%) and assigned as HEV-3f. The IAL-HEV_921 HEV-3f strain showed a close relationship to European swine, wild boar and human strains (90.7-93.2% nt), suggesting an interspecies transmission. Molecular epidemiology of HEV is poorly investigated in Brazil; subtype 3f has been reported in swine. This is the first report of HEV detected in capybara stool samples worldwide.
Databáze: MEDLINE
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