| Autor: |
Shaban AM; Biotechnology & Life Sciences Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef 62511, Egypt., Raslan M; Biotechnology & Life Sciences Department, Faculty of Postgraduate Studies for Advanced Sciences, Beni-Suef University, Beni-Suef 62511, Egypt., Sharawi ZW; Biological Sciences Department, Faculty of Sciences, King AbdulAziz University, Jeddah 21589, Saudi Arabia., Abdelhameed MS; Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef 62511, Egypt., Hammouda O; Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef 62511, Egypt., El-Masry HM; Chemistry of Natural and Microbial Products Department, Pharmaceutical and Drug Industries Research Institute, National Research Centre, Cairo 12622, Egypt., Elsayed KNM; Botany and Microbiology Department, Faculty of Science, Beni-Suef University, Beni-Suef 62511, Egypt., El-Magd MA; Anatomy Department, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafrelsheikh 33516, Egypt. |
| Jazyk: |
angličtina |
| Zdroj: |
Veterinary sciences [Vet Sci] 2023 Feb 06; Vol. 10 (2). Date of Electronic Publication: 2023 Feb 06. |
| DOI: |
10.3390/vetsci10020124 |
| Abstrakt: |
Camel milk (CM) has potent antibacterial and antifungal effects and camel milk exosomes (CM-EXO) have been shown to inhibit the proliferation of a large variety of cancer cells including HepaRG, MCF7, Hl60, and PANC1. However, little is known regarding the effects of CM-EXO on bacteria, fungi, HepG2, CaCo2, and Vero cells. Therefore, this study aimed to evaluate the antibacterial, antifungal, and anticancer effects of CM-EXO. EXOs were isolated from CM by ultracentrifugation and characterized by transmission electron microscope and flow cytometry. Unlike CM, CM-EXO (6 mg/mL) had no bactericidal effects on Gram-positive bacteria ( Staphylococcus aureus , Micrococcus luteus , and Enterococcus feacalis ) but they had bacteriostatic effects, especially against Gram-negative strains ( Escherichia coli , Pseudomonas aeruginosa , and Proteus mirabilis ), and fungistatic effects on Candida albicans . HepG2, CaCo2, and Vero cells were respectively treated with CM-EXOs at low (6.17, 3.60, 75.35 μg/mL), moderate (12.34, 7.20, 150.70 μg/mL), and high (24.68, 14.40, 301.40 μg/mL) doses and the results revealed that CM-EXOs triggered apoptosis in HepG2 and CaCo2 cells, but not in normal Vero cells, as revealed by high Bax expression and caspase 3 activities and lower expression of Bcl2 . Interestingly, CM-EXOs also induced the elevation of intracellular reactive oxygen species and downregulated the expression of antioxidant-related genes ( NrF2 and HO-1 ) in cancer cells but not in normal cells. CM-EXOs have antibacterial and antifungal effects as well as a selective anticancer effect against HepG2 and CaCo2 cells with a higher safety margin on normal cells. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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