| Autor: |
Claus MA; School of Veterinary Medicine, Murdoch University, Murdoch, WA 6150, Australia.; Perth Veterinary Specialists, Osborne Park, WA 6017, Australia., Smart L; School of Veterinary Medicine, Murdoch University, Murdoch, WA 6150, Australia.; Small Animal Specialist Hospital, Tuggerah, NSW 2259, Australia., Raisis AL; School of Veterinary Medicine, Murdoch University, Murdoch, WA 6150, Australia., Sharp CR; School of Veterinary Medicine, Murdoch University, Murdoch, WA 6150, Australia., Abraham S; School of Veterinary Medicine, Murdoch University, Murdoch, WA 6150, Australia., Gummer JPA; Forensic Sciences Laboratory, ChemCentre, Resources and Chemistry Precinct, Bentley, WA 6102, Australia.; School of Science, Edith Cowan University, Joondalup, WA 6027, Australia., Mead MK; School of Veterinary Medicine, Murdoch University, Murdoch, WA 6150, Australia., Bradley DL; Intensive Care Unit, Rockingham General Hospital, Cooloongup, WA 6168, Australia., Van Swelm R; Hepcidinanalysis.com, Department of Laboratory Medicine, Translational Metabolic Laboratory (TML 830), Radboud University Medical Center, 6525 Nijmegen, The Netherlands., Wiegerinck ETG; Hepcidinanalysis.com, Department of Laboratory Medicine, Translational Metabolic Laboratory (TML 830), Radboud University Medical Center, 6525 Nijmegen, The Netherlands., Litton E; Intensive Care Unit, Fiona Stanley Hospital, Murdoch, WA 6150, Australia.; School of Medicine, University of Western Australia, Crawley, WA 6009, Australia. |
| Abstrakt: |
Red blood cell (RBC) transfusion is associated with recipient inflammation and infection, which may be triggered by excessive circulating iron. Iron chelation following transfusion may reduce these risks. The aim of this study was to evaluate the effect of deferoxamine on circulating iron and inflammation biomarkers over time and in vitro growth of Escherichia coli ( E. coli ) following RBC transfusion in dogs with atraumatic hemorrhage. Anesthetized dogs were subject to atraumatic hemorrhage and transfusion of RBCs, then randomized to receive either deferoxamine or saline placebo of equivalent volume ( n = 10 per group) in a blinded fashion. Blood was sampled before hemorrhage and then 2, 4, and 6 h later. Following hemorrhage and RBC transfusion, free iron increased in all dogs over time (both p < 0.001). Inflammation biomarkers interleukin-6 (IL6), CXC motif chemokine-8 (CXCL8), interleukin-10 (IL10), and keratinocyte-derived chemokine (KC) increased in all dogs over time (all p < 0.001). Logarithmic growth of E. coli clones within blood collected 6 h post-transfusion was not different between groups. Only total iron-binding capacity was different between groups over time, being significantly increased in the deferoxamine group at 2 and 4 h post-transfusion (both p < 0.001). In summary, while free iron and inflammation biomarkers increased post-RBC transfusion, deferoxamine administration did not impact circulating free iron, inflammation biomarkers, or in vitro growth of E. coli when compared with placebo. |
