Cap-Independent Circular mRNA Translation Efficiency.

Autor:	Deviatkin AA; Life Sciences Research Center, Moscow Institute of Physics and Technology, National Research University, 141700 Dolgoprudniy, Russia.; Endocrinology Research Centre, 117036 Moscow, Russia., Simonov RA; Life Sciences Research Center, Moscow Institute of Physics and Technology, National Research University, 141700 Dolgoprudniy, Russia.; Martsinovsky Institute of Medical Parasitology, Tropical and Vector-Borne Diseases, First Moscow State Medical University (Sechenov University), 119991 Moscow, Russia., Trutneva KA; Life Sciences Research Center, Moscow Institute of Physics and Technology, National Research University, 141700 Dolgoprudniy, Russia.; Endocrinology Research Centre, 117036 Moscow, Russia., Maznina AA; Life Sciences Research Center, Moscow Institute of Physics and Technology, National Research University, 141700 Dolgoprudniy, Russia.; Endocrinology Research Centre, 117036 Moscow, Russia., Soroka AB; Life Sciences Research Center, Moscow Institute of Physics and Technology, National Research University, 141700 Dolgoprudniy, Russia., Kogan AA; Life Sciences Research Center, Moscow Institute of Physics and Technology, National Research University, 141700 Dolgoprudniy, Russia., Feoktistova SG; Life Sciences Research Center, Moscow Institute of Physics and Technology, National Research University, 141700 Dolgoprudniy, Russia.; Endocrinology Research Centre, 117036 Moscow, Russia., Khavina EM; Life Sciences Research Center, Moscow Institute of Physics and Technology, National Research University, 141700 Dolgoprudniy, Russia.; Faculty of Biology, Technion-Israel Institute of Technology, Haifa 32000, Israel., Mityaeva ON; Life Sciences Research Center, Moscow Institute of Physics and Technology, National Research University, 141700 Dolgoprudniy, Russia.; Endocrinology Research Centre, 117036 Moscow, Russia., Volchkov PY; Life Sciences Research Center, Moscow Institute of Physics and Technology, National Research University, 141700 Dolgoprudniy, Russia.; Endocrinology Research Centre, 117036 Moscow, Russia.
Jazyk:	angličtina
Zdroj:	Vaccines [Vaccines (Basel)] 2023 Jan 20; Vol. 11 (2). Date of Electronic Publication: 2023 Jan 20.
DOI:	10.3390/vaccines11020238
Abstrakt:	Recently, the mRNA platform has become the method of choice in vaccine development to find new ways to fight infectious diseases. However, this approach has shortcomings, namely that mRNA vaccines require special storage conditions, which makes them less accessible. This instability is due to the fact that the five-prime and three-prime ends of the mRNA are a substrate for the ubiquitous exoribonucleases. To address the problem, circular mRNAs have been proposed for transgene delivery as they lack these ends. Notably, circular RNAs do not have a capped five-prime end, which makes it impossible to initiate translation canonically. In this review, we summarize the current knowledge on cap-independent translation initiation methods and discuss which approaches might be most effective in developing vaccines and other biotechnological products based on circular mRNAs.
Databáze:	MEDLINE
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