Impedimetric nano-collision Escherichia coli analysis based on Silver-Gold bimetallic nanoparticles.

Autor: Duya CO; Department of Chemistry, Cape Peninsula University of Technology, P.O. Box 1906, Bellville, South Africa., Okumu FO; Department of Physical Sciences, Jaramogi Oginga Odinga University of Science and Technology, P. O. Box 210, 40601, Bondo, Kenya., Matoetoe MC; Department of Chemistry, Cape Peninsula University of Technology, P.O. Box 1906, Bellville, South Africa. Electronic address: lellangm@gmail.com.
Jazyk: angličtina
Zdroj: Bioelectrochemistry (Amsterdam, Netherlands) [Bioelectrochemistry] 2023 Jun; Vol. 151, pp. 108403. Date of Electronic Publication: 2023 Feb 23.
DOI: 10.1016/j.bioelechem.2023.108403
Abstrakt: An impedimetric detection of E. coli was developed using chemically synthesised bimetallic Ag-Au (1:2) nanoparticles (NPs). The UV-visible spectra of the NPs had absorption bands at 470 and 580 nm for Ag NPs and Au NPs, respectively. In the presence of E. coli, a negative potential shift and a blue shift was observed in the voltammograms and spectra respectively. The complex formed had an oxidation potential at + 0.95 V. Technique choice was based on sensitivity comparison of Differential pulse voltammetry, cyclic voltammetry and impedance spectroscopy in 0.1 M PBS with Impedance being the best choice. Optimum sensing conditions of the NPs-E. coli complex for NPs concentration, incubation period, method modulation amplitude and applied potential were 5 mM, 20 min, 10 mV and + 0.5 V, respectively. The sensor's linearity range, lower limits of detection and quantification were found to be 10 1 -10 7 , 1.88 × 10 1 , 2.34 × 10 2 cells/mL, respectively. The sensor's applicability was validated by repeatability, stability and selectivity studies showing minimum changes in signal. Potential usage of the sensor in real samples was demonstrated by standard addition analysis of sea and River water samples as well as recovery of spiked water and fruit juices with acceptable % RSD < 2%.
Competing Interests: Declaration of Competing Interest The authors declare the following financial interests/personal relationships which may be consireded as potential competing interests: [Mangaka Matoetoe reports financial support and equipment, chemicals or supplies were provided by Cape peninsula University of technology].
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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