Rapid Peak Alignment for MALDI-TOF Lipid Analysis.

Autor:	Pelit W; Chemical Biology and English, Brown University, Providence, RI, USA., de la Monte SM; Liver Research Center, Division of Gastroenterology and Department of Medicine, Rhode Island Hospital and the Alpert Medical School of Brown University, Providence, RI, USA.; Department of Pathology and Laboratory Medicine, Providence VA Medical Center and the Women & Infants Hospital of Rhode Island, Providence, RI, USA.; Departments of Neurology, Neurosurgery, and Pathology, Rhode Island Hospital and the Alpert Medical School of Brown University, Providence, RI, USA.; Division of Research, Providence VA Medical Center, Providence, RI USA., Yalcin EB; Liver Research Center, Division of Gastroenterology and Department of Medicine, Rhode Island Hospital and the Alpert Medical School of Brown University, Providence, RI, USA.; Division of Research, Providence VA Medical Center, Providence, RI USA.
Jazyk:	angličtina
Zdroj:	Journal of bioanalytical methods and techniques [J Bioanal Methods Tech] 2021; Vol. 1 (1).
Abstrakt:	The ability to measure structural and functional alterations in cellular and tissue lipids with small footprint, accessible instrumentation has sparked interest in their role in disease pathology. However, various lipidomic analytical tools tend to be cumbersome and time-consuming. A rapid, accurate, and straight forward peak alignment software routine would greatly facilitate the analysis of large datasets, such as those produced by matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Herein, we describe a novel Rapid Peak Alignment Method (RPAM) which allows untargeted analysis of lipids expressed in brain white matter following chronic ethanol exposure in an established experimental model. The RPAM outputs data comparable to manual peak alignments but the processing time requires only 90 minutes instead of 8-10 hours. This method is readily adapted to a broad range of models, tissue types, and human diseases. Competing Interests: Declaration of Conflicting Interests: The authors declare no conflicts of interest.
Databáze:	MEDLINE
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