Fecal identification markers impact the feline fecal microbiota.

Autor:	Nealon NJ; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Comparative Hepatobiliary and Intestinal Research Program, The Ohio State University, Columbus, OH, United States.; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, United States., Wood A; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Comparative Hepatobiliary and Intestinal Research Program, The Ohio State University, Columbus, OH, United States., Rudinsky AJ; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Comparative Hepatobiliary and Intestinal Research Program, The Ohio State University, Columbus, OH, United States.; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, United States., Klein H; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Comparative Hepatobiliary and Intestinal Research Program, The Ohio State University, Columbus, OH, United States.; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, United States., Salerno M; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Comparative Hepatobiliary and Intestinal Research Program, The Ohio State University, Columbus, OH, United States.; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, United States., Parker VJ; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Comparative Hepatobiliary and Intestinal Research Program, The Ohio State University, Columbus, OH, United States.; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, United States., Quimby JM; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, United States., Howard J; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Comparative Hepatobiliary and Intestinal Research Program, The Ohio State University, Columbus, OH, United States.; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, United States., Winston JA; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, Comparative Hepatobiliary and Intestinal Research Program, The Ohio State University, Columbus, OH, United States.; Department of Veterinary Clinical Sciences, College of Veterinary Medicine, The Ohio State University, Columbus, OH, United States.
Jazyk:	angličtina
Zdroj:	Frontiers in veterinary science [Front Vet Sci] 2023 Feb 08; Vol. 10, pp. 1039931. Date of Electronic Publication: 2023 Feb 08 (Print Publication: 2023).
DOI:	10.3389/fvets.2023.1039931
Abstrakt:	Fecal diagnostics are a mainstay of feline medicine, and fecal identification markers help to distinguish individuals in a multi-cat environment. However, the impact of identification markers on the fecal microbiota are unknown. Given the increased interest in using microbiota endpoints to inform diagnosis and treatment, the objective of this study was to examine the effects of orally supplemented glitter and crayon shavings on the feline fecal microbiota (amplicon sequencing of 16S rRNA gene V4 region). Fecal samples were collected daily from six adult cats that were randomized to receive oral supplementation with either glitter or crayon for two weeks, with a two-week washout before receiving the second marker. No adverse effects in response to marker supplementation were seen for any cat, and both markers were readily identifiable in the feces. Microbiota analysis revealed idiosyncratic responses to fecal markers, where changes in community structure in response to glitter or crayon could not be readily discerned. Given these findings, it is not recommended to administered glitter or crayon shavings as a fecal marker when microbiome endpoints are used, however their clinical use with other diagnostics should still be considered. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. (Copyright © 2023 Nealon, Wood, Rudinsky, Klein, Salerno, Parker, Quimby, Howard and Winston.)
Databáze:	MEDLINE
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