A new class of peptides from wasp venom: a pathway to antiepileptic/neuroprotective drugs.

Autor: Mortari MR; Neuropharmacology Laboratory, Department of Physiological Sciences, Institute of Biological Sciences, University of Brasília, Brasília 71910-900, Brazil.; Neurobiology and Venoms Laboratory, Department of Biology, Faculty of Philosophy, Sciences and Literature of Ribeirão Preto, University of São Paulo, São Paulo 14040-900, Brazil., Cunha AOS; Neurobiology and Venoms Laboratory, Department of Biology, Faculty of Philosophy, Sciences and Literature of Ribeirão Preto, University of São Paulo, São Paulo 14040-900, Brazil., Dos Anjos LC; Neuropharmacology Laboratory, Department of Physiological Sciences, Institute of Biological Sciences, University of Brasília, Brasília 71910-900, Brazil., Amaral HO; Neuropharmacology Laboratory, Department of Physiological Sciences, Institute of Biological Sciences, University of Brasília, Brasília 71910-900, Brazil., Quintanilha MVT; Neuropharmacology Laboratory, Department of Physiological Sciences, Institute of Biological Sciences, University of Brasília, Brasília 71910-900, Brazil., Gelfuso EA; Neuropharmacology Laboratory, Department of Physiological Sciences, Institute of Biological Sciences, University of Brasília, Brasília 71910-900, Brazil., Homem-de-Mello M; in Silico Toxicology Laboratory (inSiliTox), Department of Pharmacy, Health Sciences School, University of Brasilia, Brasilia 71910-900, Brazil., de Almeida H; Team CAPSID, Laboratoire Lorrain de Recherche en Informatique et ses applications (LORIA), Vandoeuvre Les Nancy F-54506, France., Rego S; Team CAPSID, Laboratoire Lorrain de Recherche en Informatique et ses applications (LORIA), Vandoeuvre Les Nancy F-54506, France., Maigret B; Team CAPSID, Laboratoire Lorrain de Recherche en Informatique et ses applications (LORIA), Vandoeuvre Les Nancy F-54506, France., Lopes NP; Organic Chemistry Laboratory, Department of Physics and Chemistry, Faculty of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, São Paulo 14040-900, Brazil., Dos Santos WF; Neurobiology and Venoms Laboratory, Department of Biology, Faculty of Philosophy, Sciences and Literature of Ribeirão Preto, University of São Paulo, São Paulo 14040-900, Brazil.
Jazyk: angličtina
Zdroj: Brain communications [Brain Commun] 2023 Feb 17; Vol. 5 (1), pp. fcad016. Date of Electronic Publication: 2023 Feb 17 (Print Publication: 2023).
DOI: 10.1093/braincomms/fcad016
Abstrakt: The ability of venom-derived peptides to disrupt physiological processes in mammals provides an exciting source for pharmacological development. Our research group has identified a new class of neuroactive peptides from the venom of a Brazilian social wasp, Polybia occidentalis , with the potential pharmacological profile to treat epilepsies. The study was divided into five phases: Phase 1 concerned the extraction, isolation and purification of Occidentalin-1202(n) from the crude venom, followed by the synthesis of an identical analogue peptide, named Occidentalin-1202(s). In Phase 2, we described the effects of both peptides in two acute models of epilepsy-kainic acid and pentylenetetrazole-induced model of seizures-and measured estimated ED 50 and therapeutic index values, electroencephalographic studies and C-fos evaluation. Phase 3 was a compilation of advanced tests performed with Occidentalin-1202(s) only, reporting histopathological features and its performance in the pilocarpine-induced status epilepticus . After the determination of the antiepileptic activity of Occidentalin-1202(s), Phase 4 consisted of evaluating its potential adverse effects, after chronic administration, on motor coordination (Rotarod) and cognitive impairment (Morris water maze) tests. Finally, in Phase 5, we proposed a mechanism of action using computational models with kainate receptors. The new peptide was able to cross the blood-brain barrier and showed potent antiseizure effects in acute (kainic acid and pentylenetetrazole) and chronic (temporal lobe epilepsy model induced by pilocarpine) models. Motor and cognitive behaviour were not adversely affected, and a potential neuroprotective effect was observed. Occidentalin-1202 can be a potent blocker of the kainate receptor, as assessed by computational analysis, preventing glutamate and kainic acid from binding to the receptor's active site. Occidentalin-1202 is a peptide with promising applicability to treat epilepsy and can be considered an interesting drug model for the development of new medicines.
(© The Author(s) 2023. Published by Oxford University Press on behalf of the Guarantors of Brain.)
Databáze: MEDLINE
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