Rapid PCR kit: lateral flow paper strip with Joule heater for SARS-CoV-2 detection.

Autor:	Kim K; Advanced Photonics Research Institute (APRI), Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea., Lee B; Department of Chemistry, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea. mkim@gist.ac.kr., Park JH; Department of Chemistry, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea. mkim@gist.ac.kr., Park JH; Department of Chemistry, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea. mkim@gist.ac.kr., Lee KJ; Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea., Kwak TJ; Center for Systems Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA., Son T; Center for Systems Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA., Shin YB; Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon 34141, Republic of Korea.; BioNano Health Guard Research Center (H-GUARD), Daejeon 34141, Republic of Korea., Im H; Center for Systems Biology, Massachusetts General Hospital, Boston, Massachusetts 02114, USA.; Department of Radiology, Massachusetts General Hospital, Boston, Massachusetts, 02114, USA., Kim MG; Department of Chemistry, Gwangju Institute of Science and Technology (GIST), Gwangju 61005, Republic of Korea. mkim@gist.ac.kr.
Jazyk:	angličtina
Zdroj:	Materials horizons [Mater Horiz] 2023 May 09; Vol. 10 (5), pp. 1697-1704. Date of Electronic Publication: 2023 May 09.
DOI:	10.1039/d2mh01267g
Abstrakt:	Polymerase chain reaction (PCR)-based diagnostic kits for point-of-care (POC) testing are highly desirable to prevent the spread of infectious diseases. Here, we demonstrate a rapid PCR testing kit that involves integrating a lateral flow paper strip with a nichrome-based thin film heater. The use of a paper membrane as a PCR-solution container results in fast thermocycling without a cooler because the membrane can contain the solution with a high specific surface area where Joule heating is applied. After PCR, amplified products are simultaneously detected at the lateral flow paper strip with the naked eye. Severe acute respiratory syndrome β-coronavirus RNA can be detected within 30 min after PCR solution injection. This work reveals that the paper membrane can act as not only a capillary flow channel but also as a promising platform for fast PCR and detection.
Databáze:	MEDLINE
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