Quantitative proteomic analysis of heifer serum at the peri-implantation period using two complementary methods.
| Autor: | Ruiz Álvarez JI; Laboratorio de Medicina Reproductiva, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina., Teijeiro JM; Laboratorio de Medicina Reproductiva, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina; and Consejo Nacional de Investigaciones Científicas y Técnicas, CONICET, Buenos Aires, Argentina., Evans C; Department Chemical and Biological Engineering, Bioanalytical Facility, University of Sheffield, Sheffield, UK., Marini PE; Laboratorio de Medicina Reproductiva, Facultad de Ciencias Bioquímicas y Farmacéuticas, Universidad Nacional de Rosario, Rosario, Argentina. |
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| Jazyk: | angličtina |
| Zdroj: | Reproduction, fertility, and development [Reprod Fertil Dev] 2023 Mar; Vol. 35 (6), pp. 416-421. |
| DOI: | 10.1071/RD22228 |
| Abstrakt: | Context: Systemic events resulting from mother-embryo relation at the peri-implantation period may result in specific changes to the protein composition of serum and thus provide a source of biomarkers for early detection of pregnancy. Aims: We set out to use two different quantitative proteomic approaches to test this hypothesis by comparing heifer serum at the peri-implantation period to that from cycling heifers. Methods: Two-dimensional electrophoresis (2-DE) and isobaric tags were used for relative and absolute quantitation (iTRAQ) of proteins. Key Results: These methods yielded complementary data indicating biomarker candidate proteins. Conclusions: Different proteomic methods provide different and complementary information that needs to be analysed in order to consider proteins as potential biomarkers. Implications: In order to characterise the proteome under specific conditions, the use of complementary techniques is advisable. |
| Databáze: | MEDLINE |
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