Thermal imprinting during embryogenesis modifies skin repair in juvenile European sea bass (Dicentrarchus labrax).

Autor: Mateus AP; Centro de Ciências do Mar (CCMAR), Comparative Endocrinology and Integrative Biology, Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal; Escola Superior de Saúde, Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal., Costa RA; Centro de Ciências do Mar (CCMAR), Comparative Endocrinology and Integrative Biology, Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal., Sadoul B; MARBEC, Univ Montpellier, CNRS, Ifremer, IRD, INRAE, 34250, Palavas-Les-Flots, France; DECOD, Ecosystem Dynamics and Sustainability, Institut Agro, Ifremer, INRAE, Rennes, France., Bégout ML; MARBEC, Univ Montpellier, CNRS, Ifremer, IRD, INRAE, 34250, Palavas-Les-Flots, France., Cousin X; MARBEC, Univ Montpellier, CNRS, Ifremer, IRD, INRAE, 34250, Palavas-Les-Flots, France., Canario AV; Centro de Ciências do Mar (CCMAR), Comparative Endocrinology and Integrative Biology, Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal; International Institution of Marine Science, Shanghai Ocean University, Shanghai, China., Power DM; Centro de Ciências do Mar (CCMAR), Comparative Endocrinology and Integrative Biology, Universidade do Algarve, Campus de Gambelas, 8005-139, Faro, Portugal; International Institution of Marine Science, Shanghai Ocean University, Shanghai, China. Electronic address: dpower@ualg.pt.
Jazyk: angličtina
Zdroj: Fish & shellfish immunology [Fish Shellfish Immunol] 2023 Mar; Vol. 134, pp. 108647. Date of Electronic Publication: 2023 Feb 25.
DOI: 10.1016/j.fsi.2023.108647
Abstrakt: Fish skin is a multifunctional tissue that develops during embryogenesis, a developmental stage highly susceptible to epigenetic marks. In this study, the impact of egg incubation temperature on the regeneration of a cutaneous wound caused by scale removal in juvenile European sea bass was evaluated. Sea bass eggs were incubated at 11, 13.5 and 16 °C until hatching and then were reared at a common temperature until 9 months when the skin was damaged and sampled at 0, 1 and 3 days after scale removal and compared to the intact skin from the other flank. Skin damage elicited an immediate significant (p < 0.001) up-regulation of pcna in fish from eggs incubated at higher temperatures. In fish from eggs incubated at 11 °C there was a significant (p < 0.001) up-regulation of krt2 compared to fish from higher thermal backgrounds 1 day after skin damage. Damaged epidermis was regenerated after 3 days in all fish irrespective of the thermal background, but in fish from eggs incubated at 11 °C the epidermis was significantly (p < 0.01) thinner compared to other groups, had less goblet cells and less melanomacrophages. The thickness of the dermis increased during regeneration of wounded skin irrespective of the thermal background and by 3 days was significantly (p < 0.01) thicker than the dermis from the intact flank. The expression of genes for ECM remodelling (mmp9, colXα, col1α1, sparc, and angptl2b) and innate immunity (lyg1, lalba, sod1, csf-1r and pparγ) changed during regeneration but were not affected by egg thermal regime. Overall, the results indicate that thermal imprinting of eggs modifies the damage-repair response in juvenile sea bass skin.
Competing Interests: Declarations of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.
(Copyright © 2023 The Authors. Published by Elsevier Ltd.. All rights reserved.)
Databáze: MEDLINE
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