An Overview of Mycoviral Curing Strategies Used in Evaluating Fungal Host Fitness.

Autor: Khan HA; Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), H-12, Islamabad, 44000, Pakistan. hkhan.phdabs13asab@asab.nust.edu.pk.; Department of Biotechnology, University of Mianwali, Punjab, 42200, Pakistan. hkhan.phdabs13asab@asab.nust.edu.pk., Baig DI; Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), H-12, Islamabad, 44000, Pakistan., Bhatti MF; Atta-ur-Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), H-12, Islamabad, 44000, Pakistan. mfbhatti@asab.nust.edu.pk.
Jazyk: angličtina
Zdroj: Molecular biotechnology [Mol Biotechnol] 2023 Oct; Vol. 65 (10), pp. 1547-1564. Date of Electronic Publication: 2023 Feb 25.
DOI: 10.1007/s12033-023-00695-1
Abstrakt: The number of novel mycoviruses is increasing at a high pace due to advancements in sequencing technologies. As a result, an uncountable number of mycoviral sequences are available in public sequence repositories. However, only genomic information is not sufficient to understand the impact of mycoviruses on their host biology. Biological characterization is required to determine the nature of mycoviruses (cryptic, hypervirulent, or hypovirulent) and to search for mycoviruses with biocontrol and therapeutic potential. Currently, no particular selective method is used as the gold standard against these mycoviral infections. Given the importance of curing, we present an overview of procedures used in preparation of isogenic lines, along with their benefits and drawbacks. We concluded that a combination of single-spore isolation and hyphal tipping is the best fit for preparation of isogenic lines. Furthermore, recent bioinformatic approaches should be introduced in the field of mycovirology to predict virus-specific antivirals to get robust results.
(© 2023. The Author(s), under exclusive licence to Springer Science+Business Media, LLC, part of Springer Nature.)
Databáze: MEDLINE
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