Alterations in the dentate gyrus of the offspring of rats treated with alprazolam during gestation.

Autor: Silva NF; Institute of Biomedical Sciences, Federal University of Uberlandia, MG, Brazil., Mascarenhas FNADP; Institute of Biomedical Sciences, Federal University of Uberlandia, MG, Brazil., Ribeiro DL; Institute of Biomedical Sciences, Federal University of Uberlandia, MG, Brazil., Zanon RG; Institute of Biomedical Sciences, Federal University of Uberlandia, MG, Brazil. Electronic address: rezanon@yahoo.com.br.
Jazyk: angličtina
Zdroj: Journal of chemical neuroanatomy [J Chem Neuroanat] 2023 Apr; Vol. 129, pp. 102253. Date of Electronic Publication: 2023 Feb 24.
DOI: 10.1016/j.jchemneu.2023.102253
Abstrakt: Benzodiazepine (BZD) abuse is a global problem, including pregnant women. For this population, the drug of choice is usually alprazolam, which acts as a GABAergic agonist and may compromise the development of integrative areas of the nervous system, such as the dentate gyrus (DG) of the hippocampus. In this context, we studied the changes in the DG of the offspring of rats treated with alprazolam during gestation: control, treatment 1 (T1: 1.25 mg/animal), and an overdose group (T2: 30 mg/animal). Alprazolam was administered orally ten days before mating and during the gestational period. After birth, newborns were counted, sexed, and the body mass of each pup was measured. The newborns' brains were extracted and processed for morphological study of the DG or for total protein extraction of the hippocampus. The results showed that alprazolam can affect the cell number and area, and increased euchromatin in both granular and molecular layers of the DG, especially in the overdose group. Also, alprazolam upregulated the NF-κB and reduced GFAP and caspase-3. Based on our findings, we conclude that the DG is a plausible region of influence by BZDs during embryogenesis. An overdose during gestation may cause structural changes in the DG.
Competing Interests: Conflict of Interest The authors have no conflicts of interest to declare.
(Copyright © 2023 Elsevier B.V. All rights reserved.)
Databáze: MEDLINE
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