| Autor: |
Baeshen NA; Department of Biological Sciences, Faculty of Science, King Abdulaziz University, Jeddah 21589, Saudi Arabia., Almulaiky YQ; Department of Chemistry, College of Science and Arts at Khulis, University of Jeddah, Jeddah 21921, Saudi Arabia.; Chemistry Department, Faculty of Applied Science, Taiz University, Taiz 3191, Yemen., Afifi M; Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.; Department of Biochemistry, College of Science, University of Jeddah, Jeddah 21959, Saudi Arabia., Al-Farga A; Department of Biochemistry, College of Science, University of Jeddah, Jeddah 21959, Saudi Arabia., Ali HA; Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt.; Department of Biochemistry, College of Science, University of Jeddah, Jeddah 21959, Saudi Arabia., Baeshen NN; Department of Biology, College of Sciences and Arts at Khulais, University of Jeddah, Jeddah 21959, Saudi Arabia., Abomughaid MM; Laboratory Medical Sciences, College of Applied Medical Sciences, University of Bisha, Bisha 61922, Saudi Arabia., Abdelazim AM; Department of Biochemistry, Faculty of Veterinary Medicine, Zagazig University, Zagazig 44519, Egypt., Baeshen MN; Department of Biology, College of Science, University of Jeddah, Jeddah 21959, Saudi Arabia. |
| Abstrakt: |
Worldwide, human beings have traditionally employed many folkloric herbal resources as complementary and alternative remedies, and these remedies have played a pivotal role in modern medicines for many decades, as scientists have used them to develop drugs. We studied the effects of employing solvents with varying polarity on the yields of phytochemical components extracted from the plant Rhazya stricta . We used chloroform-methanol (1:1), methanol, ethanol, diethyl ether, and ethyl acetate as extraction solvents. The results showed that the efficiencies of the solvents at extracting phytochemical compounds were in this order: chloroform-methanol < ethanol < methanol < diethyl ether < ethyl acetate extract. The chloroform-methanol extract produced the highest concentration of phenolic and flavonoid contents among the five solvents tested (13.3 mg GAE/g DM and 5.43 CE/g DM). The yields of the extracted phytochemical compounds ranged from 47.55 to 6.05%. The results revealed that the properties of the extraction solvents considerably impacted the extraction yield and the phytochemical components of the R. stricta extract. Furthermore, compared with the other solvents, the chloroform-methanol extraction led to the highest yield (47.55%) and to more phytochemical substances being extracted. The aim of this study is to investigate the phytochemical compounds extracted from R. stricta with different solvents that have different polarities. |
