| Autor: |
Fernández-Galleguillos C; Departamento Biomédico, Facultad de Ciencias de la Salud, Universidad de Antofagasta, Antofagasta 1270300, Chile., Jiménez-Aspee F; Institute of Nutritional Sciences, Department of Food Biofunctionality (140b), Garbenstr. 28, 70599 Stuttgart, Germany., Mieres-Castro D; Institute of Biological Sciences, University of Talca, 1 Poniente 1141, Talca 3465548, Chile., Rodríguez-Núñez YA; Departamento de Química, Facultad de Ciencias Exactas, Universidad Andrés Bello, Republica 275, Santiago 8370146, Chile., Gutiérrez M; Laboratorio de Síntesis y Actividad Biológica, Instituto de Química de Recursos Naturales, Universidad de Talca, 1 Poniente No. 1141, Talca 3460000, Chile., Guzmán L; Departamento de Bioquímica Clínica e Inmunohematología, Facultad de Ciencias de la Salud, Universidad de Talca, Talca 3460000, Chile., Echeverría J; Departamento de Ciencias del Ambiente, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago 9170022, Chile., Sandoval-Yañez C; Grupo de Investigación Química y Bioquímica Aplicada a la Biotecnología, Instituto de Ciencias Químicas Aplicadas, Facultad de Ingeniería, Universidad Autónoma de Chile, Av. Pedro de Valdivia 2541, Santiago 8320000, Chile., Forero-Doria O; Departamento de Ciencias Básicas, Facultad de Ciencias, Universidad Santo Tomás, Talca 3460000, Chile. |
| Abstrakt: |
This research aimed to identify the phenolic profile and composition of the aerial parts of three native species used in traditional medicine in the Andean Altiplano of northern Chile: Clinopodium gilliesii (Benth.) Kuntze [Lamiaceae] (commonly known as Muña-Muña), Mutisia acuminata Ruiz & Pav. var. hirsuta (Meyen) Cabrera [Asteraceae] (commonly known as Chinchircoma), and Tagetes multiflora (Kunth), [Asteraceae] (commonly known as Gracilis), as well as to evaluate their potential inhibitory effects against acetylcholinesterase (AChE) and butyrylcholinesterase (BChE). Polyphenolic enriched-extracts (PEEs) of the species were prepared and analyzed and the main components were quantified using HPLC-DAD. In total, 30 phenolic compounds were identified and quantified in all species, including simple phenolics, hydroxycinnamic acids, flavan-3-ols (monomers and polymers), flavanones, and flavonols. In addition, other main phenolics from the extracts were tentatively identified by ESI-MS-MS high-resolution analysis. T. multiflora extract showed the greatest anti-AChE and BChE activity in comparison with C. gilliesii and M. acuminata extracts, being the anti-AChE and BChE activity weak in all extracts in comparison to galantamine control. To comprise to better understand the interactions between cholinesterase enzymes and the main phenolics identified in T. multiflora , molecular docking analysis was conducted. |
