Marker-Assisted Pyramiding of Blast-Resistance Genes in a japonica Elite Rice Cultivar through Forward and Background Selection.

Autor:	Zampieri E; Council for Agricultural Research and Economics-Research Centre for Cereal and Industrial Crops, s.s. 11 to Torino, km 2.5, 13100 Vercelli, VC, Italy.; Institute for Sustainable Plant Protection, National Research Council, Strada Delle Cacce 73, 10135 Turin, TO, Italy., Volante A; Council for Agricultural Research and Economics-Research Centre for Cereal and Industrial Crops, s.s. 11 to Torino, km 2.5, 13100 Vercelli, VC, Italy.; Council for Agricultural Research and Economics-Research Centre for Vegetable and Ornamental Crops, Corso Inglesi 508, 18038 Sanremo, IM, Italy., Marè C; Council for Agricultural Research and Economics-Research Centre for Genomics and Bioinformatics, Via S. Protaso 302, 29017 Fiorenzuola d'Arda, PC, Italy., Orasen G; Bertone Sementi S.P.A., Strada Cacciolo, 15030 Terruggia, AL, Italy., Desiderio F; Council for Agricultural Research and Economics-Research Centre for Genomics and Bioinformatics, Via S. Protaso 302, 29017 Fiorenzuola d'Arda, PC, Italy., Biselli C; Council for Agricultural Research and Economics-Viticulture and Oenology, Viale Santa Margherita 80, 52100 Arezzo, AR, Italy., Canella M; Council for Agricultural Research and Economics-Research Centre for Cereal and Industrial Crops, s.s. 11 to Torino, km 2.5, 13100 Vercelli, VC, Italy., Carmagnola L; Council for Agricultural Research and Economics-Research Centre for Cereal and Industrial Crops, s.s. 11 to Torino, km 2.5, 13100 Vercelli, VC, Italy., Milazzo J; CIRAD, UMR PHIM TA A 120/K, Campus de Baillarguet, 34, CEDEX 5, 34398 Montpellier, France.; Plant Health Institute of Montpellier (PHIM), University of Montpellier, CIRAD, INRAE, IRD, Montpellier SupAgro, 34, 34398 Montpellier, France., Adreit H; CIRAD, UMR PHIM TA A 120/K, Campus de Baillarguet, 34, CEDEX 5, 34398 Montpellier, France.; Plant Health Institute of Montpellier (PHIM), University of Montpellier, CIRAD, INRAE, IRD, Montpellier SupAgro, 34, 34398 Montpellier, France., Tharreau D; CIRAD, UMR PHIM TA A 120/K, Campus de Baillarguet, 34, CEDEX 5, 34398 Montpellier, France.; Plant Health Institute of Montpellier (PHIM), University of Montpellier, CIRAD, INRAE, IRD, Montpellier SupAgro, 34, 34398 Montpellier, France., Poncelet N; CIRAD, UMR PHIM TA A 120/K, Campus de Baillarguet, 34, CEDEX 5, 34398 Montpellier, France.; Plant Health Institute of Montpellier (PHIM), University of Montpellier, CIRAD, INRAE, IRD, Montpellier SupAgro, 34, 34398 Montpellier, France., Vaccino P; Council for Agricultural Research and Economics-Research Centre for Cereal and Industrial Crops, s.s. 11 to Torino, km 2.5, 13100 Vercelli, VC, Italy., Valè G; Council for Agricultural Research and Economics-Research Centre for Cereal and Industrial Crops, s.s. 11 to Torino, km 2.5, 13100 Vercelli, VC, Italy.; Dipartimento per lo Sviluppo Sostenibile e la Transizione Ecologica, Università del Piemonte Orientale, Piazza San Eusebio 5, 13100 Vercelli, VC, Italy.
Jazyk:	angličtina
Zdroj:	Plants (Basel, Switzerland) [Plants (Basel)] 2023 Feb 08; Vol. 12 (4). Date of Electronic Publication: 2023 Feb 08.
DOI:	10.3390/plants12040757
Abstrakt:	Rice blast, caused by Pyricularia oryzae , is one of the main rice diseases worldwide. The pyramiding of blast-resistance ( Pi ) genes, coupled to Marker-Assisted BackCrossing (MABC), provides broad-spectrum and potentially durable resistance while limiting the donor genome in the background of an elite cultivar. In this work, MABC coupled to foreground and background selections based on KASP marker assays has been applied to introgress four Pi genes ( Piz, Pib, Pita , and Pik ) in a renowned japonica Italian rice variety, highly susceptible to blast. Molecular analyses on the backcross (BC) lines highlighted the presence of an additional blast-resistance gene, the Pita -linked Pita2 / Ptr gene, therefore increasing the number of blast-resistance introgressed genes to five. The recurrent genome was recovered up to 95.65%. Several lines carrying four (including Pita2 ) Pi genes with high recovery percentage levels were also obtained. Phenotypic evaluations confirmed the effectiveness of the pyramided lines against multivirulent strains, which also had broad patterns of resistance in comparison to those expected based on the pyramided Pi genes. The developed blast-resistant japonica lines represent useful donors of multiple blast-resistance genes for future rice-breeding programs related to the japonica group.
Databáze:	MEDLINE
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