The ROP2 GTPase Participates in Nitric Oxide (NO)-Induced Root Shortening in Arabidopsis.

Autor:	Kenesi E; Institute of Plant Biology, Biological Research Centre, Eötvös Lóránd Research Network, Temesvári Krt. 62, H-6726 Szeged, Hungary., Kolbert Z; Department of Plant Biology, University of Szeged, Közép Fasor 52, H-6726 Szeged, Hungary., Kaszler N; Institute of Plant Biology, Biological Research Centre, Eötvös Lóránd Research Network, Temesvári Krt. 62, H-6726 Szeged, Hungary.; Department of Plant Biology, University of Szeged, Közép Fasor 52, H-6726 Szeged, Hungary., Klement É; Laboratory of Proteomics Research, Biological Research Centre, Eötvös Lóránd Research Network, Temesvári Krt. 62, H-6726 Szeged, Hungary.; Hungarian Centre of Excellence for Molecular Medicine, Single Cell Omics ACF, H-6728 Szeged, Hungary., Ménesi D; Institute of Plant Biology, Biological Research Centre, Eötvös Lóránd Research Network, Temesvári Krt. 62, H-6726 Szeged, Hungary., Molnár Á; Department of Plant Biology, University of Szeged, Közép Fasor 52, H-6726 Szeged, Hungary., Valkai I; Institute of Plant Biology, Biological Research Centre, Eötvös Lóránd Research Network, Temesvári Krt. 62, H-6726 Szeged, Hungary., Feigl G; Department of Plant Biology, University of Szeged, Közép Fasor 52, H-6726 Szeged, Hungary., Rigó G; Institute of Plant Biology, Biological Research Centre, Eötvös Lóránd Research Network, Temesvári Krt. 62, H-6726 Szeged, Hungary., Cséplő Á; Institute of Plant Biology, Biological Research Centre, Eötvös Lóránd Research Network, Temesvári Krt. 62, H-6726 Szeged, Hungary., Lindermayr C; Institute of Biochemical Plant Pathology, Helmholtz Zentrum München-German Research Center for Environmental Health, Ingolstädter Landstraße 1, D-85764 Neuherberg, Germany., Fehér A; Institute of Plant Biology, Biological Research Centre, Eötvös Lóránd Research Network, Temesvári Krt. 62, H-6726 Szeged, Hungary.; Department of Plant Biology, University of Szeged, Közép Fasor 52, H-6726 Szeged, Hungary.
Jazyk:	angličtina
Zdroj:	Plants (Basel, Switzerland) [Plants (Basel)] 2023 Feb 08; Vol. 12 (4). Date of Electronic Publication: 2023 Feb 08.
DOI:	10.3390/plants12040750
Abstrakt:	Nitric oxide (NO) is a versatile signal molecule that mediates environmental and hormonal signals orchestrating plant development. NO may act via reversible S-nitrosation of proteins during which an NO moiety is added to a cysteine thiol to form an S-nitrosothiol. In plants, several proteins implicated in hormonal signaling have been reported to undergo S-nitrosation. Here, we report that the Arabidopsis ROP2 GTPase is a further potential target of NO-mediated regulation. The ROP2 GTPase was found to be required for the root shortening effect of NO. NO inhibits primary root growth by altering the abundance and distribution of the PIN1 auxin efflux carrier protein and lowering the accumulation of auxin in the root meristem. In rop2-1 insertion mutants, however, wild-type-like root size of the NO-treated roots were maintained in agreement with wild-type-like PIN1 abundance in the meristem. The ROP2 GTPase was shown to be S-nitrosated in vitro, suggesting that NO might directly regulate the GTPase. The potential mechanisms of NO-mediated ROP2 GTPase regulation and ROP2-mediated NO signaling in the primary root meristem are discussed.
Databáze:	MEDLINE
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