| Autor: |
Bhoopathy J; Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603103, Tamil Nadu, India., Dharmalingam S; Department of Biotechnology, College of Science and Humanities, SRM Institute of Science and Technology, Kattankulathur 603203, Tamil Nadu, India., Sathyaraj WV; Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603103, Tamil Nadu, India., Rajendran S; Centre for Nano Science and Technology, Alagappa College of Technology Campus, Anna University, Chennai 600036, Tamil Nadu, India., Rymbai S; Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam 603103, Tamil Nadu, India., Senthil R; Department of Leather Engineering, Faculty of Engineering, Ege University, 35100 Izmir, Turkey., Atchudan R; School of Chemical Engineering, Yeungnam University, Gyeongsan 38541, Republic of Korea.; Department of Chemistry, Saveetha School of Engineering, Saveetha Institute of Medical and Technical Sciences, Chennai 602105, Tamil Nadu, India. |
| Abstrakt: |
Human placenta is loaded with an enormous amount of endogenous growth factors, thereby making it a superior biomaterial for tissue regeneration. Sericin is a naturally occurring silk protein that is extensively used for biomedical applications. In the present work, sericin and human placenta-derived extracellular matrix were blended and fabricated in the form of scaffolds using the freeze-drying method for cutaneous wound treatment. The prepared sericin/placenta-derived extracellular matrix (SPEM) scaffolds were characterized to determine their morphology, functional groups, mechanical strength, and antibacterial activity. Scanning electron microscopic analysis of the scaffolds showed smooth surfaces with interconnected pores. In vitro MTT and scratch wound assays performed using HaCaT cells proved the non-toxic and wound-healing efficacy of SPEM scaffolds. In vivo CAM assay using fertilized chick embryos proved the angiogenic potency of the scaffolds. Animal experiments using Wistar albino rats proved that the open excision wounds treated with SPEM scaffolds significantly reduced wound size with collagen deposition. These results confirm that SPEM scaffolds can serve as a promising biomaterial for tissue regeneration. |
