Oxidized-Multiwalled Carbon Nanotubes as Non-Toxic Nanocarriers for Hydroxytyrosol Delivery in Cells.

Autor: Zygouri P; Department of Materials Science and Engineering, University of Ioannina, 45110 Ioannina, Greece.; Nanomedicine and Nanobiotechnology Research Group, University of Ioannina, 45110 Ioannina, Greece., Athinodorou AM; Department of Physiology, Faculty of Medicine, School of Health Sciences, University of Ioannina, 45110 Ioannina, Greece., Spyrou K; Department of Materials Science and Engineering, University of Ioannina, 45110 Ioannina, Greece.; Nanomedicine and Nanobiotechnology Research Group, University of Ioannina, 45110 Ioannina, Greece., Simos YV; Nanomedicine and Nanobiotechnology Research Group, University of Ioannina, 45110 Ioannina, Greece.; Department of Physiology, Faculty of Medicine, School of Health Sciences, University of Ioannina, 45110 Ioannina, Greece., Subrati M; Department of Materials Science and Engineering, University of Ioannina, 45110 Ioannina, Greece., Asimakopoulos G; Department of Materials Science and Engineering, University of Ioannina, 45110 Ioannina, Greece., Vasilopoulos KC; Department of Materials Science and Engineering, University of Ioannina, 45110 Ioannina, Greece., Vezyraki P; Department of Physiology, Faculty of Medicine, School of Health Sciences, University of Ioannina, 45110 Ioannina, Greece., Peschos D; Nanomedicine and Nanobiotechnology Research Group, University of Ioannina, 45110 Ioannina, Greece.; Department of Physiology, Faculty of Medicine, School of Health Sciences, University of Ioannina, 45110 Ioannina, Greece., Tsamis K; Nanomedicine and Nanobiotechnology Research Group, University of Ioannina, 45110 Ioannina, Greece.; Department of Physiology, Faculty of Medicine, School of Health Sciences, University of Ioannina, 45110 Ioannina, Greece., Gournis DP; Department of Materials Science and Engineering, University of Ioannina, 45110 Ioannina, Greece.; Nanomedicine and Nanobiotechnology Research Group, University of Ioannina, 45110 Ioannina, Greece.
Jazyk: angličtina
Zdroj: Nanomaterials (Basel, Switzerland) [Nanomaterials (Basel)] 2023 Feb 13; Vol. 13 (4). Date of Electronic Publication: 2023 Feb 13.
DOI: 10.3390/nano13040714
Abstrakt: Carbon nanotubes (CNTs) possess excellent physicochemical and structural properties alongside their nano dimensions, constituting a medical platform for the delivery of different therapeutic molecules and drug systems. Hydroxytyrosol (HT) is a molecule with potent antioxidant properties that, however, is rapidly metabolized in the organism. HT immobilized on functionalized CNTs could improve its oral absorption and protect it against rapid degradation and elimination. This study investigated the effects of cellular oxidized multiwall carbon nanotubes (oxMWCNTs) as biocompatible carriers of HT. The oxidation of MWCNTs via H 2 SO 4 and HNO 3 has a double effect since it leads to increased hydrophilicity, while the introduced oxygen functionalities can contribute to the delivery of the drug. The in vitro effects of HT, oxMWCNTS, and oxMWCNTS functionalized with HT (oxMWCNTS_HT) were studied against two different cell lines (NIH/3T3 and Tg/Tg). We evaluated the toxicity (MTT and clonogenic assay), cell cycle arrest, and reactive oxygen species (ROS) formation. Both cell lines coped with oxMWCNTs even at high doses. oxMWCNTS_HT acted as pro-oxidants in Tg/Tg cells and as antioxidants in NIH/3T3 cells. These findings suggest that oxMWCNTs could evolve into a promising nanocarrier suitable for targeted drug delivery in the future.
Databáze: MEDLINE
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