Application of Ionic Liquids as Mobile Phase Additives for Simultaneous Analysis of Nicotine and Its Metabolite Cotinine in Human Plasma by HPLC-DAD.

Autor: Axente RE; Faculty of Medicine and Pharmacy, Dunarea de Jos University of Galati, 800010 Galati, Romania., Stan M; Department of Toxicology, Carol Davila University of Medicine and Pharmacy, 20021 Bucharest, Romania., Chitescu CL; Faculty of Medicine and Pharmacy, Dunarea de Jos University of Galati, 800010 Galati, Romania., Nitescu VG; Ward ATI-Toxicology, Pediatric Clinic 2, Emergency Clinical Hospital for Children Grigore Alexandrescu, 011743 Bucharest, Romania., Vlasceanu AM; Department of Toxicology, Carol Davila University of Medicine and Pharmacy, 20021 Bucharest, Romania., Baconi DL; Department of Toxicology, Carol Davila University of Medicine and Pharmacy, 20021 Bucharest, Romania.
Jazyk: angličtina
Zdroj: Molecules (Basel, Switzerland) [Molecules] 2023 Feb 06; Vol. 28 (4). Date of Electronic Publication: 2023 Feb 06.
DOI: 10.3390/molecules28041563
Abstrakt: Nicotine and cotinine are very polar basic molecules, which makes it difficult to analyze them by reversed-phase liquid chromatography (RPLC), especially in biological samples. Additives with an ionic character have been traditionally used in RPLC as silanol suppressors. The aim of our study was to investigate the potential of selected ionic liquids in improving chromatographic performance in comparison with common additives. The experimental design was conducted using the following ionic liquids as the mobile phase modifiers: 1-butyl-3-methylimidazolium tetrafluoroborate, BMIM[BF 4 ] and 1-butyl-3-methylimidazolium hexafluorophosphate BMIM[PF 6 ], with a C18 chromatographic column. The separation of these alkaloids on silica-based RPLC stationary phases was successfully conducted by the addition of BMIM[BF 4 ] in an acetonitrile:phosphate-buffer-based mobile phase in a pH range of 2.3-5.2. The presented chromatographic method can be used as alternative for monitoring studies or pharmacokinetic application necessary for the evaluation of tobacco smoke exposure.
Databáze: MEDLINE
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje