| Autor: |
Karnachuk OV; Laboratory of Biochemistry and Molecular Biology, Tomsk State University, 634050 Tomsk, Russia., Panova IA; Laboratory of Biochemistry and Molecular Biology, Tomsk State University, 634050 Tomsk, Russia., Panov VL; Laboratory of Biochemistry and Molecular Biology, Tomsk State University, 634050 Tomsk, Russia., Ikkert OP; Laboratory of Biochemistry and Molecular Biology, Tomsk State University, 634050 Tomsk, Russia., Kadnikov VV; Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prosp, bld. 33-2, 119071 Moscow, Russia., Rusanov II; Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia., Avakyan MR; Laboratory of Biochemistry and Molecular Biology, Tomsk State University, 634050 Tomsk, Russia., Glukhova LB; Laboratory of Biochemistry and Molecular Biology, Tomsk State University, 634050 Tomsk, Russia., Lukina AP; Laboratory of Biochemistry and Molecular Biology, Tomsk State University, 634050 Tomsk, Russia., Rakitin AV; Laboratory of Biochemistry and Molecular Biology, Tomsk State University, 634050 Tomsk, Russia., Begmatov S; Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prosp, bld. 33-2, 119071 Moscow, Russia., Beletsky AV; Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prosp, bld. 33-2, 119071 Moscow, Russia., Pimenov NV; Institute of Microbiology, Research Center of Biotechnology of the Russian Academy of Sciences, 119071 Moscow, Russia., Ravin NV; Institute of Bioengineering, Research Center of Biotechnology of the Russian Academy of Sciences, Leninsky Prosp, bld. 33-2, 119071 Moscow, Russia. |
| Abstrakt: |
The diversity and activity of sulfate-reducing bacteria (SRB) in the camel gut remains largely unexplored. An abundant SRB community has been previously revealed in the feces of Bactrian camels ( Camelus bactrianus ). This study aims to combine the 16S rRNA gene profiling, sulfate reduction rate (SRR) measurement with a radioactive tracer, and targeted cultivation to shed light on SRB activity in the camel gut. Fresh feces of 55 domestic Bactrian camels grazing freely on semi-arid mountain pastures in the Kosh-Agach district of the Russian Altai area were analyzed. Feces were sampled in early winter at an ambient temperature of -15 °C, which prevented possible contamination. SRR values measured with a radioactive tracer in feces were relatively high and ranged from 0.018 to 0.168 nmol S cm -3 day -1 . The 16S rRNA gene profiles revealed the presence of Gram-negative Desulfovibrionaceae and spore-forming Desulfotomaculaceae . Targeted isolation allowed us to obtain four pure culture isolates belonging to Desulfovibrio and Desulforamulus . An active SRB community may affect the iron and copper availability in the camel intestine due to metal ions precipitation in the form of sparingly soluble sulfides. The copper-iron sulfide, chalcopyrite (CuFeS 2 ), was detected by X-ray diffraction in 36 out of 55 analyzed camel feces. In semi-arid areas, gypsum, like other evaporite sulfates, can be used as a solid-phase electron acceptor for sulfate reduction in the camel gastrointestinal tract. |
