| Autor: |
Webster J; NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute PMB 4008, Narellan, NSW 2570, Australia., Bowring B; Centre for Infectious Diseases and Microbiology, The Westmead Institute for Medical Research, Westmead, NSW 2145, Australia., Stroud L; Department of Microbiology and Infectious Diseases, Sydney South West Pathology Service, Liverpool, NSW 2170, Australia., Marsh I; NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute PMB 4008, Narellan, NSW 2570, Australia., Sales N; NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute PMB 4008, Narellan, NSW 2570, Australia., Bogema D; NSW Department of Primary Industries, Elizabeth Macarthur Agricultural Institute PMB 4008, Narellan, NSW 2570, Australia. |
| Jazyk: |
angličtina |
| Zdroj: |
Microorganisms [Microorganisms] 2023 Jan 23; Vol. 11 (2). Date of Electronic Publication: 2023 Jan 23. |
| DOI: |
10.3390/microorganisms11020297 |
| Abstrakt: |
Erysipelothrix rhusiopathiae is a bacterial pathogen that is the causative agent of erysipelas in a variety of animals, including swine, emus, turkeys, muskox, caribou, moose, and humans. This study aims to investigate the population structure and genomic features of Australian isolates of E. rhusiopathiae in the Australian pig industry and compare them to the broader scope of isolates worldwide. A total of 178 isolates (154 Australian, seven vaccine isolates, six international isolates, and 11 of unknown origin) in this study were screened against an MLST scheme and publicly available reference isolates, identifying 59 new alleles, with isolates separating into two main single locus variant groups. Investigation with BLASTn revealed the presence of the spaA gene in 171 (96%) of the isolates, with three main groups of SpaA protein sequences observed amongst the isolates. Novel SpaA protein sequences, categorised here as group 3 sequences, consisted of two sequence types forming separate clades to groups 1 and 2, with amino acid variants at positions 195 (D/A), 303 (G/E) and 323(P/L). In addition to the newly identified groups, five new variant positions were identified, 124 (S/N), 307 (Q/R), 323 (P/L), 379 (M/I), and 400 (V/I). Resistance screening identified genes related to lincomycin, streptomycin, erythromycin, and tetracycline resistance. Of the 29 isolates carrying these resistance genes, 82% belonged to SpaA group 2-N101S ( n = 22) or 2-N101S-I257L ( n = 2). In addition, 79% ( n = 23) of these 29 isolates belonged to MLST group ST 5. Our results illustrate that Australia appears to have a unique diversity of E. rhusiopathiae isolates in pig production industries within the wider global context of isolates. |
| Databáze: |
MEDLINE |
| Externí odkaz: |
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