| Autor: |
Dervisi I; Department of Botany, Faculty of Biology, National and Kapodistrian University of Athens, 15784 Athens, Greece., Petropoulos O; Department of Botany, Faculty of Biology, National and Kapodistrian University of Athens, 15784 Athens, Greece., Agalou A; Laboratory of Toxicological Control of Pesticides, Scientific Directorate of Pesticides' Control & Phytopharmacy, Benaki Phytopathological Institute (BPI), 8 Stefanou Delta Street, Kifissia, 14561 Athens, Greece., Podia V; Department of Botany, Faculty of Biology, National and Kapodistrian University of Athens, 15784 Athens, Greece., Papandreou N; Department of Cell Biology and Biophysics, Faculty of Biology, National and Kapodistrian University of Athens, 15784 Athens, Greece., Iconomidou VA; Department of Cell Biology and Biophysics, Faculty of Biology, National and Kapodistrian University of Athens, 15784 Athens, Greece., Haralampidis K; Department of Botany, Faculty of Biology, National and Kapodistrian University of Athens, 15784 Athens, Greece., Roussis A; Department of Botany, Faculty of Biology, National and Kapodistrian University of Athens, 15784 Athens, Greece. |
| Abstrakt: |
In this study, we focused on a member of the Ole e 1 domain-containing family, At SAH7, in Arabidopsis thaliana . Our lab reports for the first time on this protein, At SAH7, that was found to interact with Selenium-binding protein 1 ( At SBP1). We studied by GUS assisted promoter deletion analysis the expression pattern of AtSAH7 and determined that the sequence 1420 bp upstream of the transcription start can act as a minimal promoter inducing expression in vasculature tissues. Moreover, mRNA levels of AtSAH7 were acutely increased under selenite treatment in response to oxidative stress. We confirmed the aforementioned interaction in vivo, in silico and in planta. Following a bimolecular fluorescent complementation approach, we determined that the subcellular localization of the At SAH7 and the AtSAH7 / At SBP1 interaction occur in the ER. Our results indicate the participation of At SAH7 in a biochemical network regulated by selenite, possibly associated with responses to ROS production. |
