| Autor: |
Morsiani C; DIMEC-Department of Medicine and Surgery, University of Bologna, Via S. Giacomo 12, 40126 Bologna, Italy., Collura S; DIMEC-Department of Medicine and Surgery, University of Bologna, Via S. Giacomo 12, 40126 Bologna, Italy., Sevini F; DIMEC-Department of Medicine and Surgery, University of Bologna, Via S. Giacomo 12, 40126 Bologna, Italy.; Interdepartmental Center, Alma Mater Research Institute on Global Challenges and Climate Change (Alma Climate), University of Bologna, 40126 Bologna, Italy., Ciurca E; DIMEC-Department of Medicine and Surgery, University of Bologna, Via S. Giacomo 12, 40126 Bologna, Italy., Bertuzzo VR; Hepatobiliary and Transplant Surgery Unit, IRCCS, Universitaria Sant'Orsola Hospital, University of Bologna, 40138 Bologna, Italy., Franceschi C; Laboratory of Systems Medicine of Healthy Aging, Department of Applied Mathematics, Lobachevsky University, 603022 Nizhny Novgorod, Russia., Grazi GL; Hepatopancreatobiliary Surgery, IRCCS-Regina Elena National Cancer Institute, 00114 Rome, Italy., Cescon M; Hepatobiliary and Transplant Surgery Unit, IRCCS, Universitaria Sant'Orsola Hospital, University of Bologna, 40138 Bologna, Italy., Capri M; DIMEC-Department of Medicine and Surgery, University of Bologna, Via S. Giacomo 12, 40126 Bologna, Italy.; Interdepartmental Center, Alma Mater Research Institute on Global Challenges and Climate Change (Alma Climate), University of Bologna, 40126 Bologna, Italy. |
| Abstrakt: |
The requirement of blood-circulating sensitive biomarkers for monitoring liver transplant (LT) is currently a necessary step aiming at the reduction of standard invasive protocols, such as liver biopsy. In this respect, the main objective of this study is to assess circulating microRNA (c-miR) changes in recipients' blood before and after LT and to correlate their blood levels with gold standard biomarkers and with outcomes such as rejection or complications after graft. An miR profile was initially performed; then, the most deregulated miRs were validated by RT-qPCR in 14 recipients pre- and post-LT and compared to a control group of 24 nontransplanted healthy subjects. MiR-122-5p, miR-92a-3p, miR-18a-5p, and miR-30c-5p, identified in the validation phase, were also analyzed considering an additional 19 serum samples collected from LT recipients and focusing on different follow-up (FU) times. The results showed significant, FU-related changes in c-miRs. In particular, miR-122-5p, miR-92a-3p, and miR-18a-5p revealed the same trend after transplantation and an increase in their level was found in patients with complications, independently from FU times. Conversely, the variations in the standard haemato-biochemical parameters for liver function assessment were not significant in the same FU period, confirming the importance of c-miRs as potential noninvasive biomarkers for monitoring patients' outcomes. |
