| Autor: |
Wang Y; Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.; Jiangsu Provincial Engineering Research Center of Precision Animal Breeding, Nanjing 210014, China.; Key Laboratory of Crop and Animal Intergrated Farming, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China., Li YX; Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.; Jiangsu Provincial Engineering Research Center of Precision Animal Breeding, Nanjing 210014, China.; Key Laboratory of Crop and Animal Intergrated Farming, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China., Zhang J; Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.; Jiangsu Provincial Engineering Research Center of Precision Animal Breeding, Nanjing 210014, China.; Key Laboratory of Crop and Animal Intergrated Farming, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China., Qian Y; Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.; Jiangsu Provincial Engineering Research Center of Precision Animal Breeding, Nanjing 210014, China.; Key Laboratory of Crop and Animal Intergrated Farming, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China., Meng CH; Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.; Jiangsu Provincial Engineering Research Center of Precision Animal Breeding, Nanjing 210014, China.; Key Laboratory of Crop and Animal Intergrated Farming, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China., Zhong JF; Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.; Jiangsu Provincial Engineering Research Center of Precision Animal Breeding, Nanjing 210014, China.; Key Laboratory of Crop and Animal Intergrated Farming, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China., Cao SX; Institute of Animal Science, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China.; Jiangsu Provincial Engineering Research Center of Precision Animal Breeding, Nanjing 210014, China.; Key Laboratory of Crop and Animal Intergrated Farming, Ministry of Agriculture and Rural Affairs, Nanjing 210014, China. |
| Abstrakt: |
Sheep birth and weaning weights indicate their growth and survival. Thus, identifying molecular genetic markers for early body weight is important in sheep breeding. Pleomorphic adenoma gene 1 ( PLAG1 ) is important for regulating birth weight and body length in mammals; however, its relationship with sheep body weight remains unknown. Here, the 3'-untranslated region (3'-UTR) of the Hu sheep PLAG1 gene was cloned, single nucleotide polymorphisms (SNPs) were screened, genotype-early body weight relationships were analyzed, and the possible molecular mechanism was explored. PLAG1 3'-UTR sequences with five forms of base sequences plus poly(A) tails were detected in Hu sheep and the g.8795C>T mutation was identified. Luciferase reporter assay indicated that the g.8795C>T mutation influenced PLAG1 post-transcriptional activity. miRBase prediction showed that the g.8795C>T mutation was located in the miR-139 seed sequence binding region, and miR-139 overexpression significantly decreased both PLAG1 -CC and PLAG1 -TT activities. Moreover, the luciferase activity of PLAG1- CC was significantly lower than that of the PLAG1- TT, but miR-139 inhibition substantially increased both PLAG1 -CC and PLAG1 -TT luciferase activities, suggesting that PLAG1 is the target gene of miR-139. Thus, the g.8795C>T mutation upregulates PLAG1 expression by weakening its binding with miR-139, promoting PLAG1 expression, and increasing Hu sheep birth and weaning weights. |
