| Autor: |
Alhoqail WA; Department of Biology, College of Education, Majmaah University, Al-Majmaah 11952, Saudi Arabia., Alothaim AS; Department of Biology, College of Science in Zulfi, Majmaah University, Al-Majmaah 11952, Saudi Arabia., Suhail M; King Fahd Medical Research Center, King Abdulaziz University, Jeddah 21589, Saudi Arabia.; Department of Medical Laboratory Sciences, Faculty of Applied Medical Sciences, King Abdulaziz University, Jeddah 21589, Saudi Arabia., Iqbal D; Department of Health Information Management, College of Applied Medical Sciences, Buraydah Private Colleges, Buraydah 51418, Saudi Arabia., Kamal M; Department of Pharmaceutical Chemistry, College of Pharmacy, Prince Sattam Bin Abdulaziz University, Al-Kharj 11942, Saudi Arabia., Asmari MM; Research Center, College of Pharmacy, King Saud University, Riyadh 11451, Saudi Arabia., Jamal A; Department of Biology, College of Science in Zulfi, Majmaah University, Al-Majmaah 11952, Saudi Arabia.; Health and Basic Science Research Centre, Majmaah University, Al-Majmaah 11952, Saudi Arabia. |
| Abstrakt: |
This study effectively reports the influence of experimental incubation period on the sol-gel production of husk-like zinc oxide nanoparticles (ZNPs) and their anti-cancerous abilities. The surface morphology of ZNPs was studied with the help of SEM. With the use of TEM, the diameter range of the ZNPs was estimated to be ~86 and ~231 nm for ZNP A and ZNP B , prepared by incubating zinc oxide for 2 and 10 weeks, respectively. The X-ray diffraction (XRD) investigation showed that ZNPs had a pure wurtzite crystal structure. On prolonging the experimental incubation, a relative drop in aspect ratio was observed, displaying a distinct blue-shift in the UV-visible spectrum. Furthermore, RBC lysis assay results concluded that ZNP A and ZNP B both demonstrated innoxious nature. As indicated by MTT assay, reactive oxygen species (ROS) release, and chromatin condensation investigations against the human epidermoid carcinoma (HEC) A431 cells, ZNP B demonstrated viable relevance to chemotherapy. Compared to ZNP B , ZNP A had a slightly lower IC 50 against A431 cells due to its small size. This study conclusively describes a simple, affordable method to produce ZNP nano-formulations that display significant cytotoxicity against the skin cancer cell line A431, suggesting that ZNPs may be useful in the treatment of cancer. |
