| Autor: |
Atum ALB; Medicine Department, Universidade Nove de Julho-UNINOVE, Rua Vergueiro 249, São Paulo 01504-001, SP, Brazil., de Matos LP; Medicine Department, Universidade Nove de Julho-UNINOVE, Rua Vergueiro 249, São Paulo 01504-001, SP, Brazil., de Jesus BC; Medicine Department, Universidade Nove de Julho-UNINOVE, Rua Vergueiro 249, São Paulo 01504-001, SP, Brazil., Nasuk GR; Medicine Department, Universidade Nove de Julho-UNINOVE, Rua Vergueiro 249, São Paulo 01504-001, SP, Brazil., da Silva GA; Medicine Department, Universidade Nove de Julho-UNINOVE, Rua Vergueiro 249, São Paulo 01504-001, SP, Brazil., Gomes CP; Biophysics Department, Universidade Federal de São Paulo, Rua Pedro de Toledo 669, São Paulo 04039-032, SP, Brazil., Pesquero JB; Biophysics Department, Universidade Federal de São Paulo, Rua Pedro de Toledo 669, São Paulo 04039-032, SP, Brazil., Zamuner SR; Medicine Department, Universidade Nove de Julho-UNINOVE, Rua Vergueiro 249, São Paulo 01504-001, SP, Brazil., Silva Júnior JA; Medicine Department, Universidade Nove de Julho-UNINOVE, Rua Vergueiro 249, São Paulo 01504-001, SP, Brazil. |
| Abstrakt: |
The impact of prenatal alcohol exposure (PAE) varies considerably between individuals, leading to morphological and genetic changes. However, minor changes usually go undetected in PAE children. We investigated PAE's effects on gene transcription of genes related to cardiac dysfunction signaling in mouse myocardium and morphological changes. C57Bl/6 mice were subjected to a 10% PAE protocol. In postnatal days 2 and 60 (PN2 and PN60), morphometric measurements in the offspring were performed. Ventricular samples of the heart were collected in PN60 from male offspring for quantification of mRNA expression of 47 genes of nine myocardial signal transduction pathways related to cardiovascular dysfunction. Animals from the PAE group presented low birth weight than the Control group, but the differences were abolished in adult mice. In contrast, the mice's size was similar in PN2; however, PAE mice were oversized at PN60 compared with the Control group. Cardiac and ventricular indexes were increased in PAE mice. PAE modulated the mRNA expression of 43 genes, especially increasing the expressions of genes essential for maladaptive tissue remodeling. PAE animals presented increased antioxidant enzyme activities in the myocardium. In summary, PAE animals presented morphometric changes, transcription of cardiac dysfunction-related genes, and increased antioxidant protection in the myocardium. |
