| Autor: |
Fabresse N; Laboratory of Pharmacokinetics and Toxicology, La Timone University Hospital, 264 Rue Saint Pierre, CEDEX 5, 13385 Marseille, France., Larabi IA; Department of Pharmacology and Toxicology, Raymond Poincaré Hospital, AP-HP, 104 Boulevard Raymond Poincaré, 92380 Garches, France.; U-1018, CESP-Inserm, MOODS Team, Simone Veil Medical School, Versailles Saint-Quentin-En-Yvelines (UVSQ)-Paris-Saclay University, 2 Av. de la Source de la Bièvre, 78180 Montigny-le-Bretonneux, France., Abe E; Department of Pharmacology and Toxicology, Raymond Poincaré Hospital, AP-HP, 104 Boulevard Raymond Poincaré, 92380 Garches, France.; U-1018, CESP-Inserm, MOODS Team, Simone Veil Medical School, Versailles Saint-Quentin-En-Yvelines (UVSQ)-Paris-Saclay University, 2 Av. de la Source de la Bièvre, 78180 Montigny-le-Bretonneux, France., Lamy E; MasSpecLab, Inserm U-1173, Simone Veil Medical School, Versailles Saint-Quentin-En-Yvelines (UVSQ)-Paris-Saclay University, 2 Av. de la Source de la Bièvre, 78180 Montigny-le-Bretonneux, France., Rigothier C; Service de Néphrologie, Transplantation, Dialyse et Aphérèses, CHU de Bordeaux, 33000 Bordeaux, France., Massy ZA; Service de Néphrologie, CHU Ambroise Paré, Assistance Publique-Hôpitaux de Paris & Université Paris-Saclay (Versailles-Saint-Quentin-en-Yvelines), 9 Avenue Charles de Gaulle, 92104 Boulogne Billancourt, France.; Inserm U-1018 Centre de Recherche en Épidémiologie et Santé des Populations (CESP), Équipe 5, 94807 Villejuif, France., Alvarez JC; Department of Pharmacology and Toxicology, Raymond Poincaré Hospital, AP-HP, 104 Boulevard Raymond Poincaré, 92380 Garches, France.; U-1018, CESP-Inserm, MOODS Team, Simone Veil Medical School, Versailles Saint-Quentin-En-Yvelines (UVSQ)-Paris-Saclay University, 2 Av. de la Source de la Bièvre, 78180 Montigny-le-Bretonneux, France. |
| Abstrakt: |
The objective of the present study was to investigate the putative correlation between the saliva concentration and free serum concentration for 10 uremic toxins (UTs; eight protein-bound solutes: 3-carboxy-4-methyl-5-propyl-2-furanpropanoic acid (CMPF), hippuric acid (HA), indole-3-acetic acid (3-IAA), indoxyl sulfate (I3S), kynurenic acid (KA), kynurenine (KYN), p-cresyl glucuronide (pCG), and p-cresyl sulfate (pCS); two free, water-soluble, low-molecular weight solutes: phenylacetylglutamine (PAGN) and trimethylamine N-oxide (TMAO); and three precursors: tyrosine (Tyr), phenylalanine, and tryptophan). Saliva samples and blood samples were collected simultaneously from 18 healthy volunteers. After the addition of internal standards, 50 µL of saliva or serum were precipitated with methanol. UTs and precursors were quantified using a validated LC-MS/MS method. The saliva-serum correlation was statistically significant (according to Spearman's coefficient) for six UTs (TMAO, HA, I3S, pCS, 3-IAA, and CMPF). Tyr presented a weak saliva-serum correlation ( p = 0.08), whereas the other two precursors did not show a saliva-serum correlation. For three UTs (KYN, KA and pCG), we were unable to test the correlation since the saliva or serum levels were too low in many of the volunteers. The present study is the first to report on the saliva concentrations of TMAO, KYN, HA, PAGN, pCG, and 3-IAA. |
