Humic Acids Preparation, Characterization, and Their Potential Adsorption Capacity for Aflatoxin B 1 in an In Vitro Poultry Digestive Model.

Autor: Maguey-González JA; Posgrado en Ciencias de la Producción y de la Salud Animal, Universidad Nacional Autónoma de México (UNAM), Unidad de Posgrado, Ciudad Universitaria, Coyoacán, Ciudad de México 04510, Mexico., Nava-Ramírez MJ; Unidad de Investigación Multidisciplinaria L14 (Alimentos, Micotoxinas, y Micotoxicosis), Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México (UNAM), Cuautitlán Izcalli 54714, Mexico., Gómez-Rosales S; Centro Nacional de Investigación Disciplinaria en Fisiología y Mejoramiento Animal (CENID-INIFAP), Km1 Carretera a Colon Ajuchitlán, Querétaro 76280, Mexico., Ángeles ML; Centro Nacional de Investigación Disciplinaria en Fisiología y Mejoramiento Animal (CENID-INIFAP), Km1 Carretera a Colon Ajuchitlán, Querétaro 76280, Mexico., Solís-Cruz B; Laboratorio 5: LEDEFAR, Unidad de Investigación Multidisciplinaria, Facultad de Estudios Superiores (FES) Cuautitlán, Universidad Nacional Autónoma de México (UNAM), Cuautitlán Izcalli 54714, Mexico.; División de Ingeniería en Nanotecnología, Universidad Politécnica del Valle de México, Tultitlan 54910, Mexico., Hernández-Patlán D; Laboratorio 5: LEDEFAR, Unidad de Investigación Multidisciplinaria, Facultad de Estudios Superiores (FES) Cuautitlán, Universidad Nacional Autónoma de México (UNAM), Cuautitlán Izcalli 54714, Mexico.; División de Ingeniería en Nanotecnología, Universidad Politécnica del Valle de México, Tultitlan 54910, Mexico., Merino-Guzmán R; Departamento de Medicina y Zootecnia de Aves, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México (UNAM), Ciudad de México 04510, Mexico., Hernández-Velasco X; Departamento de Medicina y Zootecnia de Aves, Facultad de Medicina Veterinaria y Zootecnia, Universidad Nacional Autónoma de México (UNAM), Ciudad de México 04510, Mexico., Figueroa-Cárdenas JD; CINVESTAV-Unidad Querétaro, Libramiento Norponiente No. 2000, Fraccionamiento Real de Juriquilla, Querétaro 76230, Mexico., Vázquez-Durán A; Unidad de Investigación Multidisciplinaria L14 (Alimentos, Micotoxinas, y Micotoxicosis), Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México (UNAM), Cuautitlán Izcalli 54714, Mexico., Hargis BM; Department of Poultry science, University of Arkansas, Fayetteville, AR 72701, USA., Téllez-Isaías G; Department of Poultry science, University of Arkansas, Fayetteville, AR 72701, USA., Méndez-Albores A; Unidad de Investigación Multidisciplinaria L14 (Alimentos, Micotoxinas, y Micotoxicosis), Facultad de Estudios Superiores Cuautitlán, Universidad Nacional Autónoma de México (UNAM), Cuautitlán Izcalli 54714, Mexico.
Jazyk: angličtina
Zdroj: Toxins [Toxins (Basel)] 2023 Jan 17; Vol. 15 (2). Date of Electronic Publication: 2023 Jan 17.
DOI: 10.3390/toxins15020083
Abstrakt: Vermicompost was used for humic acid (HA) preparation, and the adsorption of aflatoxin B 1 (AFB 1 ) was investigated. Two forms of HA were evaluated, natural HA and sodium-free HA (SFHA). As a reference, a non-commercial zeolitic material was employed. The adsorbents were characterized by attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FTIR), energy-dispersive X-ray spectroscopy (EDS), zeta potential (ζ-potential), scanning electron microscopy (SEM), and point of zero charge (pHpzc). The adsorbent capacity of the materials when added to an AFB 1 -contaminated diet (100 µg AFB 1 /kg) was evaluated using an in vitro model that simulates the digestive tract of chickens. Characterization results revealed the primary functional groups in HA and SFHA were carboxyl and phenol. Furthermore, adsorbents have a highly negative ζ-potential at the three simulated pH values. Therefore, it appears the main influencing factors for AFB 1 adsorption are electrostatic interactions and hydrogen bonding. Moreover, the bioavailability of AFB 1 in the intestinal section was dramatically decreased when sorbents were added to the diet (0.2%, w / w ). The highest AFB 1 adsorption percentages using HA and SFHA were 97.6% and 99.7%, respectively. The zeolitic material had a considerable adsorption (81.5%). From these results, it can be concluded that HA and SFHA from vermicompost could be used as potential adsorbents to remove AFB 1 from contaminated feeds.
Databáze: MEDLINE
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