Molecular Aspects of Hypoxic Stress Effects in Chronic Ethanol Exposure of Neuronal Cells.

Autor: Stoica SI; Faculty of Medicine, University of Medicine and Pharmacy 'Carol Davila' (UMPCD), 020022 Bucharest, Romania.; Teaching Emergency Hospital 'Bagdasar-Arseni' (TEHBA), 041915 Bucharest, Romania., Onose G; Faculty of Medicine, University of Medicine and Pharmacy 'Carol Davila' (UMPCD), 020022 Bucharest, Romania.; Teaching Emergency Hospital 'Bagdasar-Arseni' (TEHBA), 041915 Bucharest, Romania., Pitica IM; Stefan S. Nicolau Institute of Virology, 285 Mihai Bravu Avenue, 030304 Bucharest, Romania., Neagu AI; Stefan S. Nicolau Institute of Virology, 285 Mihai Bravu Avenue, 030304 Bucharest, Romania., Ion G; Stefan S. Nicolau Institute of Virology, 285 Mihai Bravu Avenue, 030304 Bucharest, Romania., Matei L; Stefan S. Nicolau Institute of Virology, 285 Mihai Bravu Avenue, 030304 Bucharest, Romania., Dragu LD; Stefan S. Nicolau Institute of Virology, 285 Mihai Bravu Avenue, 030304 Bucharest, Romania., Radu LE; Stefan S. Nicolau Institute of Virology, 285 Mihai Bravu Avenue, 030304 Bucharest, Romania., Chivu-Economescu M; Stefan S. Nicolau Institute of Virology, 285 Mihai Bravu Avenue, 030304 Bucharest, Romania., Necula LG; Stefan S. Nicolau Institute of Virology, 285 Mihai Bravu Avenue, 030304 Bucharest, Romania., Anghelescu A; Faculty of Medicine, University of Medicine and Pharmacy 'Carol Davila' (UMPCD), 020022 Bucharest, Romania.; Teaching Emergency Hospital 'Bagdasar-Arseni' (TEHBA), 041915 Bucharest, Romania., Diaconu CC; Stefan S. Nicolau Institute of Virology, 285 Mihai Bravu Avenue, 030304 Bucharest, Romania., Munteanu C; Teaching Emergency Hospital 'Bagdasar-Arseni' (TEHBA), 041915 Bucharest, Romania.; Grigore T. Popa University of Medicine and Pharmacy of Iași, 700454 Iași, Romania.; Romanian Academy of Scientists, 54 Spl. Independenței Str., District 5, 050085 Bucharest, Romania., Bleotu C; Stefan S. Nicolau Institute of Virology, 285 Mihai Bravu Avenue, 030304 Bucharest, Romania.; Romanian Academy of Scientists, 54 Spl. Independenței Str., District 5, 050085 Bucharest, Romania.; Faculty of Biology, University of Bucharest, 1-3 Aleea Portocalelor Str., District 5, 060101 Bucharest, Romania.
Jazyk: angličtina
Zdroj: Current issues in molecular biology [Curr Issues Mol Biol] 2023 Feb 16; Vol. 45 (2), pp. 1655-1680. Date of Electronic Publication: 2023 Feb 16.
DOI: 10.3390/cimb45020107
Abstrakt: Experimental models of a clinical, pathophysiological context are used to understand molecular mechanisms and develop novel therapies. Previous studies revealed better outcomes for spinal cord injury chronic ethanol-consuming patients. This study evaluated cellular and molecular changes in a model mimicking spinal cord injury (hypoxic stress induced by treatment with deferoxamine or cobalt chloride) in chronic ethanol-consuming patients (ethanol-exposed neural cultures (SK-N-SH)) in order to explain the clinical paradigm of better outcomes for spinal cord injury chronic ethanol-consuming patients. The results show that long-term ethanol exposure has a cytotoxic effect, inducing apoptosis. At 24 h after the induction of hypoxic stress (by deferoxamine or cobalt chloride treatments), reduced ROS in long-term ethanol-exposed SK-N-SH cells was observed, which might be due to an adaptation to stressful conditions. In addition, the HIF-1α protein level was increased after hypoxic treatment of long-term ethanol-exposed cells, inducing fluctuations in its target metabolic enzymes proportionally with treatment intensity. The wound healing assay demonstrated that the cells recovered after stress conditions, showing that the ethanol-exposed cells that passed the acute step had the same proliferation profile as the cells unexposed to ethanol. Deferoxamine-treated cells displayed higher proliferative activity than the control cells in the proliferation-migration assay, emphasizing the neuroprotective effect. Cells have overcome the critical point of the alcohol-induced traumatic impact and adapted to ethanol (a chronic phenomenon), sustaining the regeneration process. However, further experiments are needed to ensure recovery efficiency is more effective in chronic ethanol exposure.
Databáze: MEDLINE
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