Diversity, distribution and organic substrates preferences of microbial communities of a low anthropic activity cave in North-Western Romania.

Autor:	Bogdan DF; Doctoral School of Integrative Biology, Faculty of Biology and Geology, Babeș-Bolyai University, Cluj-Napoca, Romania.; Institute for Research, Development and Innovation in Applied Natural Sciences, Cluj-Napoca, Romania., Baricz AI; Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeș-Bolyai University, Cluj-Napoca, Romania., Chiciudean I; Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeș-Bolyai University, Cluj-Napoca, Romania., Bulzu PA; Biology Centre CAS, Institute of Hydrobiology, Department of Aquatic Microbial Ecology, Laboratory of Microbial Ecology and Evolution, Ceske Budejovice, Czechia., Cristea A; Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeș-Bolyai University, Cluj-Napoca, Romania., Năstase-Bucur R; Emil Racovita Institute of Speleology, Cluj-Napoca Department, Cluj-Napoca, Romania.; Romanian Institute of Science and Technology, Cluj-Napoca, Romania., Levei EA; INCDO-INOE 2000, Research Institute for Analytical Instrumentation, Cluj-Napoca, Romania., Cadar O; INCDO-INOE 2000, Research Institute for Analytical Instrumentation, Cluj-Napoca, Romania., Sitar C; Romanian Institute of Science and Technology, Cluj-Napoca, Romania.; Zoological Museum, Babeș-Bolyai University, Cluj-Napoca, Romania., Banciu HL; Department of Molecular Biology and Biotechnology, Faculty of Biology and Geology, Babeș-Bolyai University, Cluj-Napoca, Romania.; Centre for Systems Biology, Biodiversity and Bioresources, Faculty of Biology and Geology, Babeș-Bolyai University, Cluj-Napoca, Romania., Moldovan OT; Emil Racovita Institute of Speleology, Cluj-Napoca Department, Cluj-Napoca, Romania.; Romanian Institute of Science and Technology, Cluj-Napoca, Romania.; Centro Nacional de Investigación sobre la Evolución Humana, CENIEH, Burgos, Spain.
Jazyk:	angličtina
Zdroj:	Frontiers in microbiology [Front Microbiol] 2023 Feb 07; Vol. 14, pp. 962452. Date of Electronic Publication: 2023 Feb 07 (Print Publication: 2023).
DOI:	10.3389/fmicb.2023.962452
Abstrakt:	Introduction: Karst caves are characterized by relatively constant temperature, lack of light, high humidity, and low nutrients availability. The diversity and functionality of the microorganisms dwelling in caves micro-habitats are yet underexplored. Therefore, in-depth investigations of these ecosystems aid in enlarging our understanding of the microbial interactions and microbially driven biogeochemical cycles. Here, we aimed at evaluating the diversity, abundance, distribution, and organic substrate preferences of microbial communities from Peștera cu Apă din Valea Leșului (Leșu Cave) located in the Apuseni Mountains (North-Western Romania). Materials and Methods: To achieve this goal, we employed 16S rRNA gene amplicon sequencing and community-level physiological profiling (CLPP) paralleled by the assessment of environmental parameters of cave sediments and water. Results and Discussion: Pseudomonadota (synonym Proteobacteria ) was the most prevalent phylum detected across all samples whereas the abundance detected at order level varied among sites and between water and sediment samples. Despite the general similarity at the phylum-level in Leșu Cave across the sampled area, the results obtained in this study suggest that specific sites drive bacterial community at the order-level, perhaps sustaining the enrichment of unique bacterial populations due to microenvironmental conditions. For most of the dominant orders the distribution pattern showed a positive correlation with C-sources such as putrescine, γ-amino butyric acid, and D-malic acid, while particular cases were positively correlated with polymers (Tween 40, Tween 80 and α-cyclodextrin), carbohydrates (α-D-lactose, i-erythritol, D-mannitol) and most of the carboxylic and ketonic acids. Physicochemical analysis reveals that sediments are geochemically distinct, with increased concentration of Ca, Fe, Al, Mg, Na and K, whereas water showed low nitrate concentration. Our PCA indicated the clustering of different dominant orders with Mg, As, P, Fe, and Cr. This information serves as a starting point for further studies in elucidating the links between the taxonomic and functional diversity of subterranean microbial communities. Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The reviewer FF declared a shared affiliation with the authors DFB, AIB, IC, AC, CS, and HLB to the handling editor at the time of review. (Copyright © 2023 Bogdan, Baricz, Chiciudean, Bulzu, Cristea, Năstase-Bucur, Levei, Cadar, Sitar, Banciu and Moldovan.)
Databáze:	MEDLINE
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